RRC ID |
35343
|
著者 |
Ma Y, Yu H, Pan W, Liu C, Zhang S, Shen Z.
|
タイトル |
Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant.
|
ジャーナル |
Bioresour Technol
|
Abstract |
Microbial transformation of acrylonitrile to acrylamide by nitrile hydratase is of great interest to green chemistry. During the transformation, acrylic acid is generally accumulated as a by-product through biocatalysis of amidase. A novel strain with high nitrile hydratase activity was isolated from soil and identified as Rhodococcus ruber TH by morphology and 16S rRNA gene analysis. An amidase-negative recombinant, R. ruber TH3 was constructed. Its nitrile hydratase activity was 25% higher than that of the wild type, reaching 490+/-29.2U/mg dry cell weight, while the amidase activity was 60% lower. After 6 h hydration of acrylonitrile using free cells as biocatalysts at 18 degrees C, acrylamide production by TH3 was 23% higher and the production of the by-product, acrylic acid, was 87% lower than that of the wild type. This result demonstrates that the strain TH3 could be valuable for industrial applications.
|
巻・号 |
101(1)
|
ページ |
285-91
|
公開日 |
2010-1-1
|
DOI |
10.1016/j.biortech.2009.07.057
|
PII |
S0960-8524(09)00911-0
|
PMID |
19720524
|
MeSH |
Amidohydrolases / genetics
Amidohydrolases / metabolism*
Gene Knockout Techniques
Hydro-Lyases / biosynthesis*
Mutation / genetics
Rhodococcus / genetics
Rhodococcus / isolation & purification*
Rhodococcus / metabolism*
Species Specificity
|
IF |
7.539
|
引用数 |
32
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
ENERGY & FUELS
AGRICULTURAL ENGINEERING
|
リソース情報 |
原核生物(大腸菌) |
NA |