| RRC ID |
35515
|
| 著者 |
Kiyokawa K, Yamamoto S, Sato Y, Momota N, Tanaka K, Moriguchi K, Suzuki K.
|
| タイトル |
Yeast transformation mediated by Agrobacterium strains harboring an Ri plasmid: comparative study between GALLS of an Ri plasmid and virE of a Ti plasmid.
|
| ジャーナル |
Genes Cells
|
| Abstract |
Agrobacterium strains containing a Ti plasmid can transfer T-DNA not only to plants but also to fungi, including the yeast Saccharomyces cerevisiae. However, no Agrobacterium strain harboring an Ri plasmid has been evaluated in fungal transformation. Some Ri plasmids have GALLS , instead of virE1 and virE2. GALLS protein can functionally substitute in plant transformation for a structurally different protein VirE2. In this study, we compared the yeast transformation ability among Agrobacterium donors: a strain containing a Ti plasmid, strains harboring either an agropine-type or a mikimopine-type Ri plasmid, and a strain having a modified Ri plasmid supplemented with a Ti plasmid type virE operon. Agrobacterium strains possessing GALLS transformed yeast cells far less efficiently than the strain containing virE operon. Production of GALLS in recipient yeast cells improved the yeast transformation mediated by an Agrobacterium strain lacking neither GALLS nor virE operon. A reporter assay to detect mobilization of the proteins fused with Cre recombinase revealed that VirE2 protein is much more abundant in yeast cells than GALLS. Based on these results, we concluded that the low yeast transformability mediated by Agrobacterium strains having the Ri plasmid is because of low amount of mobilized GALLS in yeast cells.
|
| 巻・号 |
17(7)
|
| ページ |
597-610
|
| 公開日 |
2012-7-1
|
| DOI |
10.1111/j.1365-2443.2012.01612.x
|
| PMID |
22686249
|
| MeSH |
Agrobacterium / genetics*
Agrobacterium / metabolism*
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Fungal
Gene Order
Operon / genetics
Plasmids / genetics*
Point Mutation
Promoter Regions, Genetic
Protein Transport
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism*
Transformation, Genetic*
|
| IF |
1.655
|
| 引用数 |
5
|
|
WOS 分野
|
GENETICS & HEREDITY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
|
