| RRC ID |
36022
|
| 著者 |
Billing O, Kao G, Naredi P.
|
| タイトル |
Mitochondrial function is required for secretion of DAF-28/insulin in C. elegans.
|
| ジャーナル |
PLoS One
|
| Abstract |
While insulin signaling has been extensively studied in Caenorhabditis elegans in the context of ageing and stress response, less is known about the factors underlying the secretion of insulin ligands upstream of the insulin receptor. Activation of the receptor governs the decision whether to progress through the reproductive lifecycle or to arrest growth and enter hibernation. We find that animals with reduced levels of the mitochondrial outer membrane translocase homologue TOMM-40 arrest growth as larvae and have decreased insulin signaling strength. TOMM-40 acts as a mitochondrial translocase in C. elegans and in its absence animals fail to import a mitochondrial protein reporter across the mitochondrial membrane(s). Inactivation of TOMM-40 evokes the mitochondrial unfolded protein response and causes a collapse of the proton gradient across the inner mitochondrial membrane. Consequently these broadly dysfunctional mitochondria render an inability to couple food abundance to secretion of DAF-28/insulin. The secretion defect is not general in nature since two other neuropeptides, ANF::GFP and INS-22::VENUS, are secreted normally. RNAi against two other putative members of the TOMM complex give similar phenotypes, implying that DAF-28 secretion is sensitive to mitochondrial dysfunction in general. We conclude that mitochondrial function is required for C. elegans to secrete DAF-28/insulin when food is abundant. This modulation of secretion likely represents an additional level of control over DAF-28/insulin function.
|
| 巻・号 |
6(1)
|
| ページ |
e14507
|
| 公開日 |
2011-1-17
|
| DOI |
10.1371/journal.pone.0014507
|
| PMID |
21264209
|
| PMC |
PMC3022011
|
| MeSH |
Animals
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism*
Insulin / metabolism*
Insulin Secretion
Insulins
Mitochondria / physiology*
Receptor, Insulin / metabolism*
|
| IF |
2.74
|
| 引用数 |
12
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 線虫 |
tm4574 |
