論文 - 詳細
RRC ID | 37151 |
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著者 | Yamazaki T, Hirai C, Ota S, Kuwano K, Kuwano S. |
タイトル | Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae. |
ジャーナル | Cryo Letters |
Abstract |
BACKGROUND AND OBJECTIVE:Cryopreservation of microorganism cultures is an important technology for their use as biological and genetic resources; however, the procedure is complicated and depends on the species. MATERIALS AND METHODS:We used the two-step freezing method for the cryopreservation of the green alga Parachlorella kessleri. RESULTS AND CONCLUSION:The optimal cryoprotectant for cryopreservation was 5% dimethyl sulfoxide plus 5% ethylene glycol. This is different from the optimal cryoprotectant for the closely related species Chlorella vulgaris. Efficient cryopreservation of P. kessleri was achieved using methanol, similar to Chlamydomonas reinhardtii. A membrane-specific fluorescent dye, FM1-43, was applied to estimate plasma membrane integrity. We found that the plasma membrane integrity of P. kessleri cells after freeze-thawing was associated with survivability, suggesting that this is a useful index for the optimization of the first step of the two-step freezing method of cryopreservation. |
巻・号 | 35(3) |
ページ | 180-7 |
公開日 | 2014-1-1 |
PMID | 24997835 |
MeSH | Cell Membrane / chemistry Chlorophyta / chemistry Chlorophyta / cytology* Cryopreservation* Cryoprotective Agents / chemistry Dimethyl Sulfoxide / chemistry Ethylene Glycol / chemistry Fluorescent Dyes / analysis* Freezing Pyridinium Compounds / analysis* Quaternary Ammonium Compounds / analysis* |
IF | 0.702 |
引用数 | 1 |
WOS 分野 | BIOLOGY PHYSIOLOGY |
リソース情報 | |
藻類 | NIES-2152 |