| RRC ID |
37581
|
| 著者 |
Ayabe T, Mizushige T, Ota W, Kawabata F, Hayamizu K, Han L, Tsuji T, Kanamoto R, Ohinata K.
|
| タイトル |
A novel Alaska pollack-derived peptide, which increases glucose uptake in skeletal muscle cells, lowers the blood glucose level in diabetic mice.
|
| ジャーナル |
Food Funct
|
| Abstract |
We found that the tryptic digest of Alaska pollack protein exhibits a glucose-lowering effect in KK-Ay mice, a type II diabetic model. We then searched for glucose-lowering peptides in the digest. Ala-Asn-Gly-Glu-Val-Ala-Gln-Trp-Arg (ANGEVAQWR) was identified from a peak of the HPLC fraction selected based on the glucose-lowering activity in an insulin resistance test using ddY mice. ANGEVAQWR (3 mg kg(-1)) decreased the blood glucose level after intraperitoneal administration. Among its fragment peptides, the C-terminal tripeptide, Gln-Trp-Arg (QWR, 1 mg kg(-1)), lowered the blood glucose level, suggesting that the C-terminal is critical for glucose-lowering activity. QWR also enhanced glucose uptake into C2C12, a mouse skeletal muscle cell line. QWR did not induce the phosphorylation of serine/threonine protein kinase B (Akt) and adenosine monophosphate-activated protein kinase (AMPK). We also demonstrated that QWR lowered the blood glucose level in NSY and KK-Ay, type II diabetic models.
|
| 巻・号 |
6(8)
|
| ページ |
2749-57
|
| 公開日 |
2015-8-1
|
| DOI |
10.1039/c5fo00401b
|
| PMID |
26152190
|
| MeSH |
Amino Acid Sequence
Animals
Blood Glucose / metabolism*
Diabetes Mellitus, Type 2 / drug therapy*
Diabetes Mellitus, Type 2 / metabolism
Fish Proteins / chemistry*
Gadiformes
Humans
Insulin / metabolism
Male
Mice
Molecular Sequence Data
Muscle, Skeletal / drug effects
Muscle, Skeletal / metabolism*
Peptides / administration & dosage*
Peptides / chemistry
|
| IF |
4.171
|
| 引用数 |
4
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
FOOD SCIENCE & TECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
