| RRC ID |
37713
|
| 著者 |
Ishikawa H, Ito S, Nishio N, Yuzawa Y, Matsuo S, Isobe K.
|
| タイトル |
Injection of bleomycin in newborn mice induces autoimmune sialitis that is transferred by CD4 T cells.
|
| ジャーナル |
Immunol Cell Biol
|
| Abstract |
Bleomycin (BLM) induces cellular apoptosis or necrosis by producing reactive oxygen species, and has been used to induce scleroderma in adult mice. We wondered whether BLM induces the same pathological phenotype in newborn mice as in adult mice. BLM was subcutaneously administrated to newborn BALB/c mice. At 1 month of age, BLM-treated mice showed severe destruction of salivary glands with enlargement of nearby lymph nodes. These nodes contained CD4(+) T cells and B220(+)cells with high expression of MHC class II molecules. In addition, autoantibodies were detected by HEp-2 staining and western blotting. The cell transfer experiments were performed to evaluate the role of autoimmune phenomena in these pathological changes. Following the transfer of enriched CD4(+) T cells to 1-month-old BALB/c nude mice, the salivary glands were severely damaged with CD4(+) T cell and B220(+) cells infiltrations. The number of T-cell antigen receptor Vbeta 8.3(+) CD4(+) T cells was significantly increased in BLM-treated murine spleen. These findings will provide new insights into the causal factors of environment in autoimmunity and the relationship between autoreactive CD4(+) T cells and autoantibodies.
|
| 巻・号 |
87(4)
|
| ページ |
351-8
|
| 公開日 |
2009-1-1
|
| DOI |
10.1038/icb.2009.1
|
| PII |
icb20091
|
| PMID |
19204734
|
| MeSH |
Adoptive Transfer
Animals
Antibiotics, Antineoplastic / administration & dosage
Antibiotics, Antineoplastic / immunology*
Autoantibodies / blood
Autoimmune Diseases / immunology*
Autoimmune Diseases / pathology
Autoimmunity / immunology
Bleomycin / administration & dosage
Bleomycin / immunology*
CD4-Positive T-Lymphocytes / drug effects
CD4-Positive T-Lymphocytes / immunology*
CD4-Positive T-Lymphocytes / transplantation
Infusions, Subcutaneous
Lymph Nodes / immunology*
Lymph Nodes / metabolism
Lymph Nodes / pathology
Mice
Mice, Inbred BALB C
Salivary Glands / immunology
Salivary Glands / pathology
Sialadenitis / immunology*
Sialadenitis / pathology
|
| IF |
3.764
|
| 引用数 |
3
|
|
WOS 分野
|
IMMUNOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HEp-2(RCB1889) |
