RRC ID |
38265
|
著者 |
Cho Y, Honda S, Yoshizawa Y, Takagaki K, Usui K, Shibuya A.
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タイトル |
Requirement of the cytoplasmic portion for dimer formation of Fcalpha/micro receptor expressed on cell surface.
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ジャーナル |
Mol Immunol
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Abstract |
Fcalpha/mu receptor (Fcalpha/muR), an Fc receptor for IgA and IgM, is the only Fc receptor for IgM identified on hematopoietic cells in human and rodents and for IgA in rodents. Fcalpha/microR is a type 1 transmembrane protein containing one immunoglobulin-like domain in the extracellular portion. Both human and mouse Fcalpha/microR mediate endocytosis of the ligands IgA and IgM, for which the cytoplasmic portion of Fcalpha/microR is responsible. However, molecular characteristics of Fcalpha/muR involved in the function have been incompletely understood. Here, we show that both monomeric and dimeric Fcalpha/microR are expressed in a mouse B cell line BCL1-B20 and BW5147 or Ba/F3 transfectants stably expressing Fcalpha/microR. We also show that the dimeric, but not monomeric, Fcalpha/microR is preferentially localized to the cell surface of the transfectants. BW5147 transfectant expressing mutant Fcalpha/microR lacking the cytoplasmic portion expressed only the monomeric Fcalpha/microR. These results suggest that the cytoplasmic portion is required for the dimer formation and thus for efficient cell surface expression of Fcalpha/microR.
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巻・号 |
47(4)
|
ページ |
878-82
|
公開日 |
2010-1-1
|
DOI |
10.1016/j.molimm.2009.10.016
|
PII |
S0161-5890(09)00788-3
|
PMID |
19945166
|
MeSH |
Animals
Cell Membrane / metabolism*
Cytoplasm / metabolism*
Humans
Ligands
Mice
Mutant Proteins / chemistry
Mutant Proteins / metabolism
Protein Multimerization*
Receptors, Fc / chemistry*
Receptors, Fc / metabolism*
Structure-Activity Relationship
Transfection
|
IF |
3.641
|
引用数 |
6
|
WOS 分野
|
IMMUNOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
|