| RRC ID |
38533
|
| 著者 |
Senju S, Haruta M, Matsumura K, Matsunaga Y, Fukushima S, Ikeda T, Takamatsu K, Irie A, Nishimura Y.
|
| タイトル |
Generation of dendritic cells and macrophages from human induced pluripotent stem cells aiming at cell therapy.
|
| ジャーナル |
Gene Ther
|
| Abstract |
This report describes generation of dendritic cells (DCs) and macrophages from human induced pluripotent stem (iPS) cells. iPS cell-derived DC (iPS-DC) exhibited the morphology of typical DC and function of T-cell stimulation and antigen presentation. iPS-DC loaded with cytomegalovirus (CMV) peptide induced vigorous expansion of CMV-specific autologous CD8+ T cells. Macrophages (iPS-MP) with activity of zymosan phagocytosis and C5a-induced chemotaxis were also generated from iPS cells. Genetically modified iPS-MPs were generated by the introduction of expression vectors into undifferentiated iPS cells, isolation of transfectant iPS cell clone and subsequent differentiation. By this procedure, we generated iPS-MP expressing a membrane-bound form of single chain antibody (scFv) specific to amyloid β (Aβ), the causal protein of Alzheimer's disease. The scFv-transfectant iPS-MP exhibited efficient Aβ-specific phagocytosis activity. iPS-MP expressing CD20-specific scFv engulfed and killed BALL-1 B-cell leukemia cells. Anti-BALL-1 effect of iPS-MP in vivo was demonstrated in a xeno-transplantation model using severe combined immunodeficient mice. In addition, we established a xeno-free culture protocol to generate iPS-DC and iPS-MP. Collectively, we demonstrated the possibility of application of iPS-DC and macrophages to cell therapy.
|
| 巻・号 |
18(9)
|
| ページ |
874-83
|
| 公開日 |
2011-9-1
|
| DOI |
10.1038/gt.2011.22
|
| PII |
gt201122
|
| PMID |
21430784
|
| MeSH |
Cell Differentiation
Cell Line, Tumor
Cell- and Tissue-Based Therapy / methods*
Dendritic Cells / cytology*
Humans
Induced Pluripotent Stem Cells / cytology*
Leukemia, B-Cell / immunology
Lymphocyte Activation
Macrophages / cytology*
Phagocytosis
Transfection
|
| IF |
4.128
|
| 引用数 |
64
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MEDICINE, RESEARCH & EXPERIMENTAL
BIOCHEMISTRY & MOLECULAR BIOLOGY
GENETICS & HEREDITY
|
| リソース情報 |
| ヒト・動物細胞 |
BALL-1(RCB0256)
OP9(RCB1124) |
