RRC ID |
386
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著者 |
Reim G, Mizoguchi T, Stainier DY, Kikuchi Y, Brand M.
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タイトル |
The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova.
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ジャーナル |
Dev Cell
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Abstract |
The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation of sox17 by casanova (cas). We report that spg (pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygotic spg function (MZspg) lack endodermal precursors. Cell transplantations show that spg acts in early endodermal precursors, and cas mRNA-injection into MZspg embryos does not restore endoderm development. spg and cas together are both necessary and sufficient to activate endoderm development, and stimulate expression of a sox17 promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation by spg and cas suggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development.
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巻・号 |
6(1)
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ページ |
91-101
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公開日 |
2004-1-1
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DOI |
10.1016/s1534-5807(03)00396-4
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PII |
S1534-5807(03)00396-4
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PMID |
14723850
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MeSH |
Animals
Body Patterning / genetics
Cell Differentiation / genetics
Cell Lineage / genetics
DNA-Binding Proteins / genetics
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / embryology*
Embryo, Nonmammalian / metabolism
Endoderm / cytology
Endoderm / metabolism*
Female
Gene Expression Regulation, Developmental / genetics
High Mobility Group Proteins / genetics
High Mobility Group Proteins / metabolism*
Male
Octamer Transcription Factor-3
Promoter Regions, Genetic / genetics
RNA, Messenger / pharmacology
SOX Transcription Factors
SOXF Transcription Factors
Stem Cells / cytology
Stem Cells / metabolism
Transcription Factors / deficiency*
Transcription Factors / genetics
Transcription Factors / metabolism*
Zebrafish / embryology*
Zebrafish / metabolism
Zebrafish Proteins*
Zygote / cytology
Zygote / metabolism
|
IF |
10.092
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引用数 |
88
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WOS 分野
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DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
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リソース情報 |
ゼブラフィッシュ |
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