論文 - 詳細
RRC ID | 38632 |
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著者 | Takai T, Ohmori H. |
タイトル | DNA transfection of mouse lymphoid cells by the combination of DEAE-dextran-mediated DNA uptake and osmotic shock procedure. |
ジャーナル | Biochim Biophys Acta |
Abstract |
Several mouse lymphoid cell lines were efficiently transfected with plasmid DNA by a novel method combining DEAE-dextran-mediated DNA uptake and osmotic shock procedure. The cells were first incubated with DNA-DEAE-dextran complex, treated with hypertonic Tris-HCl buffer containing 0.5 M sucrose and 10% poly(ethylene glycol), and then exposed to hypotonic RPMI 1640 medium. This transfection protocol exhibited maximal frequencies of 0.3% and 3.10(-5) for transient gene expression and stable transformation in P3-NSI/1-Ag4-1 cells, respectively. |
巻・号 | 1048(1) |
ページ | 105-9 |
公開日 | 1990-1-30 |
DOI | 10.1016/0167-4781(90)90029-2 |
PII | 0167-4781(90)90029-2 |
PMID | 2297528 |
MeSH | Animals Cell Line DEAE-Dextran DNA / genetics* Electrophoresis, Agar Gel Gene Expression Mice Osmotic Pressure Transfection* |
IF | 3.411 |
引用数 | 69 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | P3/NSI/1-AG4-1(RCB0095) |