| RRC ID |
38734
|
| Author |
Suzuki H, Takahashi K, Yasumoto K, Shibahara S.
|
| Title |
Activation of the tyrosinase gene promoter by neurofibromin.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Neurofibromatosis type 1 (NF1) is a hereditary cancer syndrome, characterized by café-au-lait skin spots and multiple neurofibromas. A gene linked to NF1 encodes neurofibromin, an established function of which is to stimulate intrinsic GTPase activity of ras protein. By transiently coexpressing a neurofibromin cDNA in a melanoma cell line, we show that neurofibromin increases the expression of a reporter gene under the control of the tyrosinase gene promoter. Tyrosinase is a rate-limiting enzyme in melanin biosynthesis and is expressed only in melanin-producing cells. Functional analysis of neurofibromin cDNAs suggests that the domain related to a GTPase-activating protein is mainly responsible for this induction. These results suggest that neurofibromin functions as a regulator of melanogenesis, a process specific to the melanocytes derived from the neural crest.
|
| Volume |
205(3)
|
| Pages |
1984-91
|
| Published |
1994-12-30
|
| DOI |
10.1006/bbrc.1994.2903
|
| PII |
S0006-291X(84)72903-2
|
| PMID |
7811291
|
| MeSH |
Animals
Cell Line
Cloning, Molecular
DNA, Complementary / genetics
Gene Expression Regulation, Enzymologic / drug effects
Genes, Neurofibromatosis 1
HeLa Cells
Humans
Monophenol Monooxygenase / genetics*
Neurofibromatosis 1 / enzymology
Neurofibromatosis 1 / genetics
Neurofibromin 1
Promoter Regions, Genetic / drug effects*
Proteins / genetics
Proteins / pharmacology*
Rats
|
| IF |
2.985
|
| Times Cited |
18
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007)
HMV-II(RCB0777) |
