| RRC ID |
38860
|
| 著者 |
Ohuchi E, Azumano I, Yoshida S, Iwata K, Okada Y.
|
| タイトル |
A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 7 (matrilysin) using monoclonal antibodies.
|
| ジャーナル |
Clin Chim Acta
|
| Abstract |
A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 7 (MMP-7, matrilysin, EC 3.4.24.23) was established with a pair of monoclonal antibodies prepared against the zymogen of MMP-7 (proMMP-7) purified from CaR-1 human rectal carcinoma cells and against an oligopeptide corresponding to the C-terminal domain of human proMMP-7. ProMMP-7 in samples was allowed to simultaneously react with both solid-phase and peroxidase-labeled antibodies. Sensitivity of the EIA system in the presence of EDTA was 0.05 microgram/l (1.5 pg/assay) and linearity was obtained between 0.156-10 micrograms/l (4.68-300 pg/assay). ProMMP-7 levels in human sera from healthy subjects were shown to be in the range of 10.7 +/- 18.8 micrograms/l. Gel filtration analyses indicated that proMMP-7 makes polymers after treatment with EDTA and suggested that proMMP-7 exists as polymers in normal human sera.
|
| 巻・号 |
244(2)
|
| ページ |
181-98
|
| 公開日 |
1996-1-31
|
| DOI |
10.1016/0009-8981(95)06199-1
|
| PII |
0009898195061991
|
| PMID |
8714436
|
| MeSH |
Amino Acid Sequence
Antibodies, Monoclonal
Cell Line
Chromatography, Gel
Colonic Neoplasms / blood*
Colonic Neoplasms / enzymology
Colonic Neoplasms / pathology
Electrophoresis, Polyacrylamide Gel
Enzyme Precursors / blood
Humans
Immunoblotting
Immunoenzyme Techniques
Matrix Metalloproteinase 7
Metalloendopeptidases / blood*
Molecular Sequence Data
Molecular Weight
Neoplasm Staging
Peptide Fragments / chemical synthesis
Peptide Fragments / immunology
Rectal Neoplasms
Reference Values
Sensitivity and Specificity
Tumor Cells, Cultured
|
| IF |
2.615
|
| 引用数 |
31
|
|
WOS 分野
|
MEDICAL LABORATORY TECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NB1RGB(RCB0222) |
