| RRC ID |
38925
|
| 著者 |
Miyazaki Y, Shinomura Y, Higashiyama S, Kanayama S, Higashimoto Y, Tsutsui S, Zushi S, Taniguchi N, Matsuzawa Y.
|
| タイトル |
Heparin-binding EGF-like growth factor is an autocrine growth factor for rat gastric epithelial cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
We examined the biological action and expression of heparin-binding EGF-like growth factor (HB-EGF) in a rat gastric mucosal cell line, RGM1. HB-EGF stimulated DNA synthesis of RGM1 cells in a dose-dependent manner. Mitogenic effect of HB-EGF was as potent as that of other known mitogens for gastric epithelial cells, such as hepatocyte growth factor (HGF) and transforming growth factor (TGF)-alpha. Northern blot analysis showed that RGM1 cells as well as rat gastric mucosal tissue expressed a 2.5-kilobase transcript of HB-EGF. Not only HB-EGF and TGF-alpha but also HGF caused a rapid induction of HB-EGF mRNA in the cells. Treatment with heparitinase which destroys heparan sulfate proteoglycan (HSPG) or with chlorate which inhibits sulfation of HSPG diminished [3H]thymidine incorporation of RGM1 cells in serum-free medium. In addition, a synthetic peptide corresponding to the heparin-binding domain of HB-EGF inhibits the DNA synthesis of RGM1 cells in serum-free medium in a dose-dependent manner. These results suggest that HB-EGF is an autocrine and paracrine growth factor for gastric epithelial cells and may play significant roles in mucosal repair of the stomach in cooperation with other growth factors.
|
| 巻・号 |
223(1)
|
| ページ |
36-41
|
| 公開日 |
1996-6-5
|
| DOI |
10.1006/bbrc.1996.0842
|
| PII |
S0006-291X(96)90842-6
|
| PMID |
8660375
|
| MeSH |
Amino Acid Sequence
Animals
Blotting, Northern
Cell Division / drug effects
Cell Line
Culture Media, Serum-Free
DNA / biosynthesis
DNA / drug effects
Epidermal Growth Factor / biosynthesis*
Epidermal Growth Factor / pharmacology*
Gastric Mucosa / cytology
Gastric Mucosa / drug effects
Gastric Mucosa / metabolism*
Heparin / metabolism
Heparin-binding EGF-like Growth Factor
Hepatocyte Growth Factor / pharmacology
Intercellular Signaling Peptides and Proteins
Kinetics
Molecular Sequence Data
Peptide Fragments / chemical synthesis
Peptide Fragments / pharmacology
RNA, Messenger / biosynthesis
Rats
Rats, Wistar
Thymidine / metabolism
Transcription, Genetic / drug effects
Transforming Growth Factor alpha / pharmacology
|
| IF |
2.985
|
| 引用数 |
25
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RGM1(RCB0876) |
