論文 - 詳細
| RRC ID | 38949 |
|---|---|
| 著者 | Maeda Y, Ueda H, Kazami J, Kawano G, Suzuki E, Nagamune T. |
| タイトル | Truncation of Vargula luciferase still results in retention of luminescence. |
| ジャーナル | J Biochem |
| Abstract |
Significant amino acid sequence homology in two regions of Vargula hilgendorfii to one in apoaequorin was reported. The intra-amino acid homology in Vargula luciferase between residues 81-312 and 321-540 was 19.3%, and each of this intra-homologous region contained the region homologous to apoaequorin. In order to prove the possibility that only one of the homologous regions is sufficient for luminescence, we have produced a chimeric protein comprising of only the N-terminal homologous region of Vargula luciferase fused to protein A. Comparison of the luminescence of this truncate luciferase indicated that there was 38.5% retention in the bioluminescence of luciferase when compared to that of the mature form of luciferase. This fact may have interesting implications for further study of engineering luciferase. |
| 巻・号 | 119(4) |
| ページ | 601-3 |
| 公開日 | 1996-4-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a021284 |
| PMID | 8743557 |
| MeSH | Animals Base Sequence Cell Line Chlorocebus aethiops Crustacea / enzymology* Crustacea / genetics Genes / genetics Luciferases / analysis Luciferases / biosynthesis Luciferases / genetics* Luciferases / metabolism Luminescent Measurements* Molecular Sequence Data Recombinant Fusion Proteins / biosynthesis Sequence Deletion / genetics* Sequence Homology, Amino Acid Staphylococcal Protein A / genetics |
| IF | 2.476 |
| 引用数 | 2 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | COS-1(RCB0143) |