Reference - Detail
| RRC ID | 39152 |
|---|---|
| Author | Higuchi A, Kao SH, Ling QD, Chen YM, Li HF, Alarfaj AA, Munusamy MA, Murugan K, Chang SC, Lee HC, Hsu ST, Kumar SS, Umezawa A. |
| Title | Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity. |
| Journal | Sci Rep |
| Abstract |
The tentative clinical application of human pluripotent stem cells (hPSCs), such as human embryonic stem cells and human induced pluripotent stem cells, is restricted by the possibility of xenogenic contamination resulting from the use of mouse embryonic fibroblasts (MEFs) as a feeder layer. Therefore, we investigated hPSC cultures on biomaterials with different elasticities that were grafted with different nanosegments. We prepared dishes coated with polyvinylalcohol-co-itaconic acid hydrogels grafted with an oligopeptide derived from vitronectin (KGGPQVTRGDVFTMP) with elasticities ranging from 10.3 to 30.4 kPa storage moduli by controlling the crosslinking time. The hPSCs cultured on the stiffest substrates (30.4 kPa) tended to differentiate after five days of culture, whereas the hPSCs cultured on the optimal elastic substrates (25 kPa) maintained their pluripotency for over 20 passages under xeno-free conditions. These results indicate that cell culture matrices with optimal elasticity can maintain the pluripotency of hPSCs in culture. |
| Volume | 5 |
| Pages | 18136 |
| Published | 2015-12-14 |
| DOI | 10.1038/srep18136 |
| PII | srep18136 |
| PMID | 26656754 |
| PMC | PMC4677349 |
| MeSH | Amino Acid Sequence Cell Culture Techniques / methods* Cell Differentiation* Cell Line Cell Proliferation* Culture Media / chemistry Elastic Modulus Humans Hydrogels / chemistry* Immunohistochemistry Molecular Sequence Data Octamer Transcription Factor-3 / metabolism Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism Polyvinyl Alcohol / chemistry Reproducibility of Results SOXB1 Transcription Factors / metabolism Succinates / chemistry Time Factors Vitronectin / chemistry Xenobiotics / chemistry |
| IF | 3.998 |
| Times Cited | 29 |
| WOS Category | MATERIALS SCIENCE, BIOMATERIALS |
| Resource | |
| Human and Animal Cells | 454E2(HPS0077) |