| RRC ID |
39278
|
| 著者 |
Takaoka AS, Yamada T, Gotoh M, Kanai Y, Imai K, Hirohashi S.
|
| タイトル |
Cloning and characterization of the human beta4-integrin gene promoter and enhancers.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
The cell-surface adhesion molecule alpha6beta4-integrin is a receptor for laminins and a component of hemidesmosomes. beta4-Integrin expression is restricted to proliferating basal keratinocytes in the epidermis and is suppressed when differentiation commences. Altered beta4-integrin expression levels correlate significantly with the aggressive behavior of cancers. In order to clarify the mechanisms that regulate transcription of the beta4-integrin gene, we cloned its 5'-flanking region. This 5'-flanking region was found to have a high G + C content and not to contain either TATA or CAAT boxes. Nested delimitation and reporter analyses mapped a basal promoter to nucleotides -106 to +105, surrounding the most proximal transcription initiation site. Gel retardation and mutational analyses revealed that cooperation between AP1 and Ets, interacting with other factors, mediated the promoter activity. In addition to the promoter element, enhancer activity was found in the first intron (+1905/+3933) and in a sequence upstream of the promoter region (-414/-107). These findings should facilitate our understanding of the regulation of beta4-integrin gene expression in processes such as cell growth and differentiation, apoptosis, and cancer development and metastasis.
|
| 巻・号 |
273(50)
|
| ページ |
33848-55
|
| 公開日 |
1998-12-11
|
| DOI |
10.1074/jbc.273.50.33848
|
| PII |
S0021-9258(19)88608-8
|
| PMID |
9837976
|
| MeSH |
Antigens, CD / genetics*
Base Sequence
Cloning, Molecular
DNA
Enhancer Elements, Genetic*
Exons
Gene Expression Regulation
Humans
Integrin beta4
Introns
Molecular Sequence Data
Promoter Regions, Genetic*
Protein Binding
RNA, Messenger / genetics
Transcription Factors / metabolism
Transcription, Genetic
|
| IF |
4.238
|
| 引用数 |
38
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NB1RGB(RCB0222)
WI-38(RCB0702)
HFL-I(RCB0521) |
