RRC ID |
39280
|
著者 |
Matsuya M, Sasaki H, Aoto H, Mitaka T, Nagura K, Ohba T, Ishino M, Takahashi S, Suzuki R, Sasaki T.
|
タイトル |
Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions.
|
ジャーナル |
J Biol Chem
|
Abstract |
Cell adhesion kinase beta (CAKbeta/PYK2) is the second protein-tyrosine kinase of the focal adhesion kinase subfamily. We identified a cDNA that encodes a CAKbeta-binding protein. This cDNA clone encodes the human homologue of Hic-5, the cDNA of which was cloned in 1994 as transforming growth factor beta1- and hydrogen peroxide-inducible mRNA. We found that Hic-5 exclusively localized at focal adhesions in a rat fibroblast line, WFB. This localization of Hic-5 was confirmed in WFB cells expressing Myc-tagged Hic-5. The amino acid sequence of Hic-5 is highly similar to that of paxillin in the four LD motifs as well as in the four contiguous LIM domains. The Hic-5 N-terminal domain directly associated in vitro with the extreme C-terminal region (residue 801 to the end) of CAKbeta. CAKbeta was coimmunoprecipitated with Hic-5 from the WFB cell lysate. The coimmunoprecipitation of CAKbeta with Hic-5 was markedly inhibited by the addition of the extreme C-terminal region of CAKbeta. Coimmunoprecipitation of Hic-5 with CAKbeta, which was shown in COS-7 cells doubly transfected with cDNA constructs of CAKbeta and Myc-tagged Hic-5, was lost when the CAKbeta amino acid residues 741-903 were deleted. Hic-5 was tyrosine-phosphorylated in Src-transformed 3Y1 cells and in cells treated with pervanadate. Hic-5 associated with CAKbeta was selectively tyrosine-phosphorylated in WFB cells exposed to hypertonic osmotic stress. These results indicate that Hic-5 is a paxillin-related component of focal adhesions and binds to CAKbeta, implying possible involvement of Hic-5 in the downstream signaling of CAKbeta.
|
巻・号 |
273(2)
|
ページ |
1003-14
|
公開日 |
1998-1-9
|
DOI |
10.1074/jbc.273.2.1003
|
PII |
S0021-9258(18)39569-3
|
PMID |
9422762
|
MeSH |
Amino Acid Sequence
Animals
COS Cells
Cell Line
Child, Preschool
Cloning, Molecular
Cytoskeletal Proteins / chemistry
DNA, Complementary
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Female
Focal Adhesion Kinase 2
Glutathione Transferase / genetics
Humans
Intracellular Signaling Peptides and Proteins
LIM Domain Proteins
Lysophospholipids / pharmacology
Molecular Sequence Data
Oxidative Stress
Paxillin
Phosphoproteins / chemistry
Precipitin Tests
Protein-Tyrosine Kinases / genetics
Protein-Tyrosine Kinases / metabolism*
Rats
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Sequence Homology, Amino Acid
|
IF |
4.238
|
引用数 |
111
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
|