| RRC ID |
39333
|
| 著者 |
Sawada H, Murakami M, Enomoto A, Shimbara S, Kudo I.
|
| タイトル |
Regulation of type V phospholipase A2 expression and function by proinflammatory stimuli.
|
| ジャーナル |
Eur J Biochem
|
| Abstract |
Types IIA and V secretory phospholipase A2 (sPLA2) are structurally related to each other and their genes are tightly linked to the same chromosome locus. An emerging body of evidence suggests that sPLA2-IIA plays an augmentative role in long-term prostaglandin (PG) generation in cells activated by proinflammatory stimuli; however, the mechanism underlying the functional regulation of sPLA2-V remains largely unknown. Here we show that sPLA2-V is more widely expressed than sPLA2-IIA in the mouse, in which its expression is elevated by proinflammatory stimuli such as lipopolysaccharide. In contrast, proinflammatory stimuli induced sPLA2-IIA in marked preference to sPLA2-V in the rat. Cotransfection of sPLA2-V with cyclooxygenase (COX)-2, but not with COX-1, into human embryonic kidney 293 cells dramatically increased the interleukin-1-dependent PGE2 generation occurring over a 24 h of culture period. Rat mastocytoma RBL-2H3 cells overexpressing sPLA2-V exhibited increased IgE-dependent PGD2 generation and accelerated beta-hexosaminidase exocytosis. These results suggest that sPLA2-V acts as a regulator of inflammation-associated cellular responses. This possible compensation of sPLA2-V for sPLA2-IIA in many, if not all, tissues may also explain why some mouse strains with natural disruption of the sPLA2-IIA gene exhibit few abnormalities during their life-spans.
|
| 巻・号 |
263(3)
|
| ページ |
826-35
|
| 公開日 |
1999-8-1
|
| DOI |
10.1046/j.1432-1327.1999.00565.x
|
| PII |
ejb565
|
| PMID |
10469147
|
| MeSH |
Animals
Bone Marrow Cells / cytology
Cell Line
Dinoprostone / metabolism
Exocytosis
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Enzymologic / physiology*
Heparin / pharmacology
Humans
Inflammation
Interleukin-1 / pharmacology
Isoenzymes
Kidney
Lipopolysaccharides / pharmacology*
Mast Cells / enzymology
Mast-Cell Sarcoma
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Phospholipases A / biosynthesis
Phospholipases A / genetics*
Phospholipases A2
Prostaglandin D2 / metabolism
Rats
Rats, Wistar
Recombinant Proteins / biosynthesis
Transfection
Tumor Cells, Cultured
beta-N-Acetylhexosaminidases / metabolism
|
| 引用数 |
77
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RBL 2H3(RCB2782) |
