| RRC ID |
39408
|
| 著者 |
Tanaka T, Mizuno K, Miyama M, Chang L, Chen H, Ohtaki S, Umesaki N, Ogita S.
|
| タイトル |
Enhanced Fas/CD95-mediated apoptosis by epidermal growth factor in human endometrial epithelial cells.
|
| ジャーナル |
Eur J Obstet Gynecol Reprod Biol
|
| Abstract |
Epidermal growth factor (EGF) has been reported to regulate apoptosis in various cell lineages. Throughout the menstrual cycle overexpression of the EGF receptor in the secretory epithelium and constitutive expression of EGF in all types of endometrial cells were identified by immunohistochemical study of normal human endometrial tissues. However, it is not known whether EGF also regulates endometrial apoptosis. This study examined the regulatory functions of EGF in endometrial apoptosis by using a human endometrial epithelial cell line HHUA which is susceptible to Fas-mediated apoptosis. Although EGF alone did not affect the cell growth of HHUA, EGF pretreatment of HHUA enhanced Fas-mediated growth suppression and Fas-mediated DNA fragmentation in the cells. Flowcytometric analyses demonstrated that EGF did not induce Fas expression on the cell surface while expressions of the EGF receptor were down-regulated. These results suggest that EGF may enhance apoptotic susceptibility of the endometrial epithelium, especially in the secretory epithelium.
|
| 巻・号 |
86(2)
|
| ページ |
189-94
|
| 公開日 |
1999-10-1
|
| DOI |
10.1016/s0301-2115(99)00048-2
|
| PII |
S0301211599000482
|
| PMID |
10509790
|
| MeSH |
Apoptosis*
Cell Division / drug effects
Cell Line
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Down-Regulation
Endometrium / cytology
Endometrium / drug effects*
Endometrium / metabolism
Epidermal Growth Factor / biosynthesis
Epidermal Growth Factor / pharmacology*
Epidermal Growth Factor / physiology
Epithelial Cells / cytology
Epithelial Cells / drug effects
Epithelial Cells / metabolism
ErbB Receptors / biosynthesis
Female
Flow Cytometry
Humans
Immunoglobulin M / pharmacology
Immunohistochemistry
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
Stromal Cells / metabolism
fas Receptor / biosynthesis
fas Receptor / immunology
fas Receptor / physiology*
|
| IF |
1.868
|
| 引用数 |
13
|
|
WOS 分野
|
OBSTETRICS & GYNECOLOGY
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HHUA(RCB0658) |
