論文 - 詳細
| RRC ID | 39539 |
|---|---|
| 著者 | Fukunaga T, Nagahama M, Hatsuzawa K, Tani K, Yamamoto A, Tagaya M. |
| タイトル | Implication of sphingolipid metabolism in the stability of the Golgi apparatus. |
| ジャーナル | J Cell Sci |
| Abstract |
We examined the effects of short chain and long chain ceramides on the stability of the Golgi apparatus. Short chain ceramides, C(2)- and C(6)-ceramides, blocked brefeldin A-induced Golgi disassembly without affecting the rapid release of Golgi coat proteins, whereas they did not inhibit brefeldin A-induced tubulation of endosomes. Both short chain ceramides also retarded Golgi disassembly induced by nordihydroguaiaretic acid and nocodazole, suggesting that they stabilize the Golgi apparatus. In contrast to short chain ceramides, natural long chain ceramides, when incorporated into cells or formed within cells upon treatment with sphingomyelinase or metabolic inhibitors, enhanced brefeldin A-induced Golgi disassembly. These results suggest that sphingolipid metabolism is implicated in the stability of the Golgi apparatus. |
| 巻・号 | 113 ( Pt 18) |
| ページ | 3299-307 |
| 公開日 | 2000-9-1 |
| DOI | 10.1242/jcs.113.18.3299 |
| PMID | 10954427 |
| MeSH | Animals Brefeldin A / pharmacology Cattle Cell Line Ceramides / metabolism* Ceramides / pharmacology Coat Protein Complex I / metabolism Endocannabinoids Endosomes / metabolism Enzyme Inhibitors / pharmacology Ethanolamines / pharmacology Golgi Apparatus / drug effects Golgi Apparatus / metabolism Golgi Apparatus / physiology* Golgi Apparatus / ultrastructure Masoprocol / pharmacology Microscopy, Electron Morpholines / pharmacology Nocodazole / pharmacology Oleic Acids Sphingolipids / biosynthesis Sphingolipids / metabolism* Sphingomyelin Phosphodiesterase / antagonists & inhibitors Sphingosine / analogs & derivatives* Sphingosine / metabolism Sphingosine / pharmacology trans-Golgi Network / drug effects trans-Golgi Network / physiology trans-Golgi Network / ultrastructure |
| IF | 4.573 |
| 引用数 | 25 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | NRK(RCB0043) |