| RRC ID |
39609
|
| 著者 |
Koike J, Nagata K, Kudo S, Tsuji T, Irimura T.
|
| タイトル |
Density-dependent induction of TNF-alpha release from human monocytes by immobilized P-selectin.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
P-selectin purified from human platelets, when immobilized on a solid surface, induced monocytes to release tumor necrosis factor-alpha (TNF-alpha). The induction of TNF-alpha release was dependent on the concentration of P-selectin used for the immobilization, and the maximal stimulation was observed when the plate was coated with 0.3 microgram/ml of P-selectin. Use of either a higher or a lower concentration of P-selectin for the plate-coating was found to elicit less TNF-alpha release, although the higher concentration of P-selectin caused a stronger adhesion of HL-60 leukemic cells. The expression of mRNA for TNF-alpha roughly paralleled the TNF-alpha secretion, as assessed by RT-PCR. These results indicate that monocytes are activated by immobilized P-selectin in a density-dependent manner.
|
| 巻・号 |
477(1-2)
|
| ページ |
84-8
|
| 公開日 |
2000-7-14
|
| DOI |
10.1016/s0014-5793(00)01765-8
|
| PII |
S0014-5793(00)01765-8
|
| PMID |
10899315
|
| MeSH |
Antibodies, Monoclonal
Blood Platelets / chemistry
Cell Adhesion / drug effects
Cell Count
Cells, Cultured
Dose-Response Relationship, Drug
HL-60 Cells
Humans
Kinetics
Lipopolysaccharides / pharmacology
Molecular Weight
Monocytes / cytology
Monocytes / drug effects*
Monocytes / metabolism*
P-Selectin / chemistry
P-Selectin / isolation & purification
P-Selectin / metabolism*
P-Selectin / pharmacology*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / metabolism*
|
| IF |
3.057
|
| 引用数 |
30
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60RCB0041)
L·P3(RCB0101) |
