論文 - 詳細
| RRC ID | 39774 |
|---|---|
| 著者 | Suzuki H, Mori M, Seto K, Shibata F, Nagahashi S, Kawaguchi C, Suzuki M, Matsui H, Watanabe K, Miura S, Ishii H. |
| タイトル | Rat CXC chemokine GRO/CINC-1 paradoxically stimulates the growth of gastric epithelial cells. |
| ジャーナル | Aliment Pharmacol Ther |
| Abstract |
BACKGROUND:CXC chemokines such as interleukin (IL)-8 are neutrophil chemoattractants, the levels of which increase in Helicobacter pylori-infected gastric mucosa. Many investigators have focused on the chemotactic aspects of IL-8: however, CXC chemokines are also reported to have angiogenic activity and to serve as remodelling factors. Rat GRO/CINC-1 is a rodent counterpart of human GROalpha, a member of the family of CXC chemokines. Gastric mucosa infected with H. pylori is in a state of hyperproliferation, with increases in the amounts of growth factors such as hepatocyte growth factor (HGF). AIM:To investigate whether rat GRO/CINC-1 had growth-stimulating activity for gastric epithelial cells. METHODS:The rat gastric epithelial cell line RGM-1 was incubated in serum-free medium for 12 h to adjust the cell cycle to the G0 phase, and GRO/CINC-1 was then added for 24 h. The total cell number was determined by fluorogenic analysis after propidium iodide staining, and cell proliferation was assessed by measuring 5-bromo-2'-deoxyuridine (BrdU) incorporation. The activity of p42/p44 mitogen-activated protein kinase (MAPK) was measured 5-20 min after the start of GRO/CINC-1 exposure. RESULTS:Cultures treated with GRO/CINC-1 showed a significant increase in cell number and BrdU incorporation in a concentration-dependent fashion. The MAPK activity increased within 5 min after GRO/CINC-1 application and returned to the control level at 20 min. CONCLUSION:The growth-stimulatory effect of GRO/CINC-1 on rat gastric epithelial cells suggests a dual function of this chemokine: proinflammatory action and induction of epithelial proliferation. |
| 巻・号 | 14 Suppl 1 |
| ページ | 94-100 |
| 公開日 | 2000-4-1 |
| DOI | 10.1046/j.1365-2036.2000.014s1094.x |
| PMID | 10807410 |
| MeSH | Animals Autoradiography Bromodeoxyuridine Cell Division / drug effects Cell Line Chemokines, CXC / pharmacology* Epithelial Cells / physiology* Gastric Mucosa / cytology* Gastric Mucosa / drug effects Inflammation Rats |
| IF | 7.515 |
| 引用数 | 15 |
| WOS 分野 | PHARMACOLOGY & PHARMACY GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |