論文 - 詳細
| RRC ID | 39814 |
|---|---|
| 著者 | Mori H, Arai T, Hirota K, Ishii H, Endo N, Makino K, Fukuda K. |
| タイトル | Effects of 6-formylpterin, a xanthine oxidase inhibitor and a superoxide scavenger, on production of nitric oxide in RAW 264.7 macrophages. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
As well as superoxide generated from neutrophils, nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) in macrophages plays an important role in inflammation. We previously showed that 6-formylpterin, a xanthine oxidase inhibitor, has a superoxide scavenging activity. In the present study, to elucidate other pharmacological activities of 6-formylpterin, we investigated the effects of 6-formylpterin on production of nitric oxide (NO) in the murine macrophage cell line RAW 264.7 stimulated by lipopolysaccharide (LPS) and interferon-gamma (INF-gamma). 6-Formylpterin suppressed the expression of iNOS, and it also inhibited the catalytic activity of iNOS, which collectively resulted in the inhibition of NO production in the stimulated macrophages. However, 6-formylpterin did not scavenge the released NO from an NO donor, S-nitroso-N-acetylpenicillamine (SNAP). These results indicate that 6-formylpterin inhibits pathological NO generation from macrophages during inflammation, but that it does not disturb the physiological action of NO released from other sources. |
| 巻・号 | 1474(1) |
| ページ | 93-9 |
| 公開日 | 2000-3-6 |
| DOI | 10.1016/s0304-4165(99)00210-x |
| PII | S0304-4165(99)00210-X |
| PMID | 10699495 |
| MeSH | Animals Anti-Inflammatory Agents, Non-Steroidal / pharmacology Cell Line Cell Survival / drug effects Cyclooxygenase 2 Electron Spin Resonance Spectroscopy Free Radical Scavengers / pharmacology Interferon-gamma Isoenzymes / genetics Isoenzymes / metabolism Lipopolysaccharides Luciferases Macrophages / drug effects* Macrophages / metabolism Mice Molecular Structure Nitric Oxide / biosynthesis* Nitric Oxide Synthase / genetics Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type II Prostaglandin-Endoperoxide Synthases / genetics Prostaglandin-Endoperoxide Synthases / metabolism Pteridines / chemical synthesis Pteridines / pharmacology* Pterins* RNA, Messenger / metabolism Spin Trapping Superoxides / metabolism* Xanthine Oxidase / antagonists & inhibitors |
| IF | 3.411 |
| 引用数 | 8 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |