論文 - 詳細
| RRC ID | 39890 |
|---|---|
| 著者 | Ikeda S, Hachisu R, Yamaguchi A, Gao YH, Okano T. |
| タイトル | Radiation retards muscle differentiation but does not affect osteoblastic differentiation induced by bone morphogenetic protein-2 in C2C12 myoblasts. |
| ジャーナル | Int J Radiat Biol |
| Abstract |
PURPOSE:Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS:A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was estimated by counting the number of myosin heavy chain (MHC)-positive multinucleated myotubes. Osteoblast differentiation was analysed by measuring alkaline phosphatase (ALP) activity and osteocalcin production in C2C12 cells treated with recombinant human bone morphogenetic protein-2 (rhBMP-2). RESULTS:Myotube formation in C2C12 cells was inhibited by 2 and 4 Gy irradiation, but recovered to non-irradiated control levels following further culture. In irradiated cultures, the number of MHC-positive mononucleated cells increased significantly. Irradiation did not, however, alter the induction of osteoblastic phenotypes induced by rhBMP-2 in C2C12. Irradiation also did not affect the expression of MyoD mRNA and myogenin mRNA in myogenic differentiation and induction of osteocalcin mRNA in osteoblastic differentiation. CONCLUSIONS:During differentiation of C2C12 cells, myotube formation was delayed after irradiation, while induction of osteoblasts with BMP-2 was unaffected. |
| 巻・号 | 76(3) |
| ページ | 403-11 |
| 公開日 | 2000-3-1 |
| DOI | 10.1080/095530000138745 |
| PMID | 10757320 |
| MeSH | Alkaline Phosphatase / biosynthesis Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / metabolism* Bone Morphogenetic Proteins / pharmacology Cell Differentiation / drug effects Cell Differentiation / radiation effects* Cell Line Humans Mice Muscle, Skeletal / cytology Muscle, Skeletal / drug effects Muscle, Skeletal / metabolism* Muscle, Skeletal / radiation effects* Myogenin / biosynthesis Myogenin / genetics Myosin Heavy Chains / biosynthesis Myosins / biosynthesis Myosins / genetics Osteoblasts / cytology Osteoblasts / enzymology* Osteocalcin / biosynthesis Osteocalcin / genetics RNA, Messenger / biosynthesis Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Transforming Growth Factor beta* |
| IF | 2.368 |
| 引用数 | 21 |
| WOS 分野 | NUCLEAR SCIENCE & TECHNOLOGY RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |