Reference - Detail
| RRC ID | 39916 |
|---|---|
| Author | Kohda A, Taguchi H, Okumura K. |
| Title | Visualization of biallelic expression of the imprinted SNRPN gene induced by inhibitors of DNA methylation and histone deacetylation. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
We have adapted a fluorescence in situ hybridization (FISH) method to detect nascent RNA molecules of the imprinted SNRPN gene at the initial transcription sites in nuclei of human HL60 and WI38 cells. Simultaneous detection of RNA and DNA of SNRPN by FISH using the cosmid probe confirmed its monoallelic expression in these cell lines. Treatment of both cells by inhibitors of DNA methylation and histone deacetylation resulted in time-dependent increase of the cell population with the biallelic expression of SNRPN. |
| Volume | 65(5) |
| Pages | 1236-9 |
| Published | 2001-5-1 |
| DOI | 10.1271/bbb.65.1236 |
| PMID | 11440148 |
| MeSH | Acetylation Alleles* Autoantigens / genetics* DNA Methylation* Gene Expression Regulation / drug effects* Genomic Imprinting* HL-60 Cells Histone Deacetylases / metabolism Histones / metabolism Humans In Situ Hybridization, Fluorescence Ribonucleoproteins, Small Nuclear* snRNP Core Proteins |
| IF | 1.516 |
| Times Cited | 2 |
| WOS Category | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | HL60(RCB0041) WI-38 |