RRC ID 40778
著者 Ueki A, Shibuya T, Kaku N, Ueki K.
タイトル Aminocella lysinolytica gen. nov., sp. nov., a L-lysine-degrading, strictly anaerobic bacterium in the class Clostridia isolated from a methanogenic reactor of cattle farms.
ジャーナル Arch Microbiol
Abstract A strictly anaerobic bacterial strain (WN037(T)) was isolated from a methanogenic reactor. Cells were Gram-positive rods. Strain WN037(T) was asaccharolytic. The strain fermented L-lysine in the presence of B-vitamin mixture or vitamin B12 and produced acetate and butyrate. L-arginine and casamino acids poorly supported the growth. Strain WN037(T) used neither other amino acids nor organic acids examined. The strain had C18:1 ω7c, C16:0 and C18:1 ω7c DMA as the predominant cellular fatty acids. The genomic DNA G + C content was 44.2 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain WN037(T) in the family Eubacteriaceae in the class Clostridia. The closest relative was Eubacterium pyruvativorans (sequence similarity, 92.8 %). Based on the comprehensive analyses, the novel genus and species, Aminocella lysinolytica gen. nov., sp. nov. was proposed to accommodate the strain. The type strain is WN037(T) (= JCM 19863(T) = DSM 28287(T)).
巻・号 197(1)
ページ 97-104
公開日 2015-1-1
DOI 10.1007/s00203-014-1066-z
PMID 25449329
MeSH Amino Acids / metabolism Animals Bacteria, Anaerobic / classification* Bacteria, Anaerobic / cytology Bacteria, Anaerobic / isolation & purification* Bacteria, Anaerobic / physiology Base Composition Bioreactors* Cattle Culture Media DNA, Bacterial / genetics Fatty Acids / analysis Fermentation Genes, rRNA Gram-Positive Bacteria / classification* Gram-Positive Bacteria / cytology Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / physiology Lysine / metabolism* Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 1.884
引用数 3
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19863