RRC ID |
40778
|
著者 |
Ueki A, Shibuya T, Kaku N, Ueki K.
|
タイトル |
Aminocella lysinolytica gen. nov., sp. nov., a L-lysine-degrading, strictly anaerobic bacterium in the class Clostridia isolated from a methanogenic reactor of cattle farms.
|
ジャーナル |
Arch Microbiol
|
Abstract |
A strictly anaerobic bacterial strain (WN037(T)) was isolated from a methanogenic reactor. Cells were Gram-positive rods. Strain WN037(T) was asaccharolytic. The strain fermented L-lysine in the presence of B-vitamin mixture or vitamin B12 and produced acetate and butyrate. L-arginine and casamino acids poorly supported the growth. Strain WN037(T) used neither other amino acids nor organic acids examined. The strain had C18:1 ω7c, C16:0 and C18:1 ω7c DMA as the predominant cellular fatty acids. The genomic DNA G + C content was 44.2 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain WN037(T) in the family Eubacteriaceae in the class Clostridia. The closest relative was Eubacterium pyruvativorans (sequence similarity, 92.8 %). Based on the comprehensive analyses, the novel genus and species, Aminocella lysinolytica gen. nov., sp. nov. was proposed to accommodate the strain. The type strain is WN037(T) (= JCM 19863(T) = DSM 28287(T)).
|
巻・号 |
197(1)
|
ページ |
97-104
|
公開日 |
2015-1-1
|
DOI |
10.1007/s00203-014-1066-z
|
PMID |
25449329
|
MeSH |
Amino Acids / metabolism
Animals
Bacteria, Anaerobic / classification*
Bacteria, Anaerobic / cytology
Bacteria, Anaerobic / isolation & purification*
Bacteria, Anaerobic / physiology
Base Composition
Bioreactors*
Cattle
Culture Media
DNA, Bacterial / genetics
Fatty Acids / analysis
Fermentation
Genes, rRNA
Gram-Positive Bacteria / classification*
Gram-Positive Bacteria / cytology
Gram-Positive Bacteria / isolation & purification*
Gram-Positive Bacteria / physiology
Lysine / metabolism*
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
IF |
1.884
|
引用数 |
3
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 19863 |