RRC ID |
41483
|
著者 |
Masuoka M, Yoshimuta T, Hamada M, Okamoto M, Fumimori T, Honda J, Oizumi K, Itoh K.
|
タイトル |
Identification of the HLA-A24 peptide epitope within cytomegalovirus protein pp65 recognized by CMV-specific cytotoxic T lymphocytes.
|
ジャーナル |
Viral Immunol
|
Abstract |
Among cytomegalovirus (CMV) tegument proteins, phosphoprotein 65 (pp65) has been identified as the important target antigen of the cytotoxic T lymphocyte (CTL) response against the virus. We synthesized seven CMV-pp65-derived peptides carrying an HLA-A24-binding motif, and investigated the ability of these peptides to induce CMV-specific CTL. We identified one nonamer peptide (pp65113-121; VYALPLKML) able to bind HLA-A24 and induce CTL responses in vitro in peripheral blood mononuclear cells (PBMC) from CMV-seropositive individuals. The peptide-specific CTLs generated were capable of recognizing pp65 expressed on CMV-infected fibroblasts as well as pp65113-121 peptide bound to the surface of C1R-A*2402 cells in an HLA-A24-restricted manner. The pp65113-121 peptide thus might be considered a synthetic peptide vaccine in HLA-A24-positive individuals.
|
巻・号 |
14(4)
|
ページ |
369-77
|
公開日 |
2001-1-1
|
DOI |
10.1089/08828240152716619
|
PMID |
11792066
|
MeSH |
Antigens, Viral / immunology
Cells, Cultured
Cytomegalovirus / immunology*
Epitopes, T-Lymphocyte / immunology*
HLA-A Antigens / analysis*
HLA-A Antigens / immunology
HLA-A24 Antigen
Humans
Peptides / chemical synthesis
Peptides / immunology
Phosphoproteins / chemical synthesis
Phosphoproteins / immunology*
T-Lymphocytes, Cytotoxic / immunology*
Viral Matrix Proteins / chemical synthesis
Viral Matrix Proteins / immunology*
|
IF |
1.716
|
引用数 |
26
|
WOS 分野
|
IMMUNOLOGY
VIROLOGY
|
リソース情報 |
ヒト・動物細胞 |
MRC-5 |