RRC ID |
41547
|
著者 |
Suda M, Tanaka K, Yasoda A, Komatsu Y, Chusho H, Miura M, Tamura N, Ogawa Y, Nakao K.
|
タイトル |
C-type natriuretic peptide/guanylate cyclase B system in ATDC5 cells, a chondrogenic cell line.
|
ジャーナル |
J Bone Miner Metab
|
Abstract |
Natriuretic peptides constitute a family of three structurally related peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). Particulate guanylate cyclases, GC-A, and GC-B, are the receptors for these peptides to mediate their action. ANP and BNP possess high affinities for GC-A, and CNP is the preferred ligand for GC-B. In this article, we report our study of the expression and possible role(s) of natriuretic peptides in ATDC5 cells, which represent a chondrogenic cell line. ATDC5 cells produced cyclic guanosine monophosphate (cGMP) in response to natriuretic peptides. CNP was far more potent than ANP in terms of cGMP production. The messages for GC-A and GC-B were demonstrated by means of Northern blot analysis, and the presence of CNP was shown by Southern blotting coupled with reverse transcription-polymerase chain reaction (RT-PCR). These results suggest that the CNP/GC-B system is preferentially expressed in ATDC5 cells. GC-B mRNA expression was higher at 14 days after confluency than that at confluency. CNP or 8-bromo cGMP reduced [3H] thymidine uptake and slightly increased the message for collagen type X, which is a marker of hypertrophic chondrocytes. These data suggest that the CNP/GC-B system is likely to be an autocrine/paracrine regulator of ATDC5 cells, thus affecting both their growth and differentiation.
|
巻・号 |
20(3)
|
ページ |
136-41
|
公開日 |
2002-1-1
|
DOI |
10.1007/s007740200019
|
PMID |
11984695
|
MeSH |
Animals
Blotting, Northern
Blotting, Southern
Cell Differentiation
Cell Line
Chondrocytes / cytology
Chondrocytes / metabolism*
Collagen Type II / analysis
Collagen Type II / metabolism
Collagen Type X / analysis
Collagen Type X / metabolism
Cyclic GMP / analogs & derivatives
Cyclic GMP / metabolism
DNA Replication / drug effects
DNA Replication / physiology
Guanylate Cyclase / metabolism*
Mice
Natriuretic Peptide, C-Type / genetics
Natriuretic Peptide, C-Type / metabolism*
Natriuretic Peptide, C-Type / pharmacology
PC12 Cells
RNA, Messenger / analysis
RNA, Messenger / biosynthesis
Rats
Receptors, Parathyroid Hormone / genetics
Receptors, Parathyroid Hormone / metabolism
Thymidine / analogs & derivatives
Thymidine / metabolism
|
IF |
2.297
|
引用数 |
23
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
ENDOCRINOLOGY & METABOLISM
|
リソース情報 |
ヒト・動物細胞 |
ATDC5(RCB0565) |