| RRC ID |
41670
|
| 著者 |
Kato T, Katabami K, Takatsuki H, Han SA, Takeuchi K, Irimura T, Tsuji T.
|
| タイトル |
Characterization of the promoter for the mouse alpha 3 integrin gene.
|
| ジャーナル |
Eur J Biochem
|
| Abstract |
The alpha 3 beta 1 integrin is an adhesion receptor for extracellular matrix proteins including isoforms of laminin, and the changes of its expression level in various cancer cells are thought to cause their malignant phenotypes. We have cloned an approximately 4 kb DNA fragment of the 5'-flanking region of the murine alpha 3 integrin gene and analyzed its promoter activity. Transfection of MKN1 gastric carcinoma cells with serially truncated segments of the 5'-flanking region linked to a luciferase gene indicated that a 537-bp SalI/SacI fragment upstream of exon 1 was sufficient to promote high level gene expression. By 5'-rapid amplification of cDNA ends (5'-RACE) using a cap site-labeled cDNA library, we determined one major and one minor transcription start sites in this region. The murine alpha 3 integrin gene was found to contain a CCAAT box, but to lack a TATA box. Luciferase assay following transfection with a series of deletion constructs of the SalI/SacI fragment revealed that the sequence between positions -260 and -119 bp (relative to the major transcription start site) is required for efficient transcription in gastric carcinoma cells. The sequence analysis of this segment showed the presence of several consensus sequences for transcription factors including Ets, GATA and MyoD/E-box binding factors. The introduction of mutation in one of the Ets-binding sequences greatly decreased its promoter activity, suggesting that the transcription of the alpha 3 integrin gene in these cells is regulated by the Ets-family of transcription factors.
|
| 巻・号 |
269(18)
|
| ページ |
4524-32
|
| 公開日 |
2002-9-1
|
| DOI |
10.1046/j.1432-1033.2002.03146.x
|
| PII |
3146
|
| PMID |
12230564
|
| MeSH |
5' Flanking Region
Amino Acid Sequence
Animals
Base Sequence
DNA, Neoplasm
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Neoplastic*
Humans
Integrin alpha3beta1 / genetics*
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Mutation
Promoter Regions, Genetic*
Sequence Analysis, DNA
Sequence Deletion
Transcription Factors / metabolism*
Transcription Initiation Site
Tumor Cells, Cultured
|
| 引用数 |
20
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MKN1(RCB1003)
MKN28(RCB1000)
MKN45(RCB1001) |
