| RRC ID |
41793
|
| 著者 |
Nakashima Y, Yano M, Kobayashi Y, Moriyama S, Sasaki H, Toyama T, Yamashita H, Fukai I, Iwase H, Yamakawa Y, Fujii Y.
|
| タイトル |
Endostatin gene therapy on murine lung metastases model utilizing cationic vector-mediated intravenous gene delivery.
|
| ジャーナル |
Gene Ther
|
| Abstract |
Tumors require ongoing angiogenesis to support their growth. Inhibition of angiogenesis by production of antiangiogenic factors should be a viable approach for cancer gene therapy. In this study, we investigated whether intravenous administration of endostatin gene complexed with a cationic vector (GL67/DOPE or PEI22K) could inhibit the development of lung tumors in mice injected i.v. with NFSa Y83 fibrosarcoma cells (5 x 10(5)) which frequently form lung metastasis. mRNA and protein of the transfected gene were produced in the lung and other organs of the transfected mice as assessed by immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction. Single intravenous injection of the endostatin gene (60 microg) complexed with either GL67/DOPE or PEI22K on day 3 or day 7 after fibrosarcoma cell inoculation significantly inhibited tumor formation in the lung as evidenced by the reduced number of lung tumors and lung weight, and prolonged survival of the endostatin gene-transfected mice compared with control mice. These findings suggested that the endostatin gene therapy, using cationic vector-mediated intravenous gene transfer, might be a feasible strategy for organ-targeted prevention and regulation of possible disseminated cancers.
|
| 巻・号 |
10(2)
|
| ページ |
123-30
|
| 公開日 |
2003-1-1
|
| DOI |
10.1038/sj.gt.3301856
|
| PII |
3301856
|
| PMID |
12571641
|
| MeSH |
Animals
COS Cells
Collagen / analysis
Collagen / genetics*
Endostatins
Fibrosarcoma / pathology
Fibrosarcoma / secondary*
Fibrosarcoma / therapy*
Gene Expression
Genetic Therapy / methods*
Genetic Vectors / administration & dosage
Immunohistochemistry / methods
Injections, Intravenous
Lung Neoplasms / pathology
Lung Neoplasms / secondary*
Lung Neoplasms / therapy*
Mice
Mice, Inbred C3H
Models, Animal
Neovascularization, Pathologic
Peptide Fragments / analysis
Peptide Fragments / genetics*
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Transfection / methods
Tumor Cells, Cultured
|
| IF |
4.128
|
| 引用数 |
20
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
