| RRC ID |
41821
|
| 著者 |
Matsuguchi T, Takagi A, Matsuzaki T, Nagaoka M, Ishikawa K, Yokokura T, Yoshikai Y.
|
| タイトル |
Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activities in macrophages through Toll-like receptor 2.
|
| ジャーナル |
Clin Diagn Lab Immunol
|
| Abstract |
Lactobacilli are nonpathogenic gram-positive inhabitants of microflora. At least some Lactobacillus strains have been postulated to have health beneficial effects, such as the stimulation of the immune system. Here we examined the stimulatory effects of lactobacilli on mouse immune cells. All six heat-killed Lactobacillus strains examined induced the secretion of tumor necrosis factor alpha (TNF-alpha) from mouse splenic mononuclear cells, albeit to various degrees. When fractionated subcellular fractions of Lactobacillus casei were tested for NF-kappaB activation and TNF-alpha production in RAW264.7, a mouse macrophage cell line, the activity was found to be as follows: protoplast > cell wall > polysaccharide-peptidoglycan complex. Both crude extracts and purified lipoteichoic acids (LTAs) from two Lactobacillus strains, L. casei and L. fermentum, significantly induced TNF-alpha secretion from RAW264.7 cells and splenocytes of C57BL/6, C3H/HeN, and C3H/HeJ mice but not from splenocytes of C57BL/6 TLR2(-/-) mice. Lactobacillus LTA induced activation of c-Jun N-terminal kinase activation in RAW264.7 cells. Furthermore, in HEK293T cells transected with a combination of CD14 and Toll-like receptor 2 (TLR2), NF-kappaB was activated in response to Lactobacillus LTA. Taken together, these data suggest that LTAs from lactobacilli elicit proinflammatory activities through TLR2.
|
| 巻・号 |
10(2)
|
| ページ |
259-66
|
| 公開日 |
2003-3-1
|
| DOI |
10.1128/cdli.10.2.259-266.2003
|
| PMID |
12626452
|
| PMC |
PMC150522
|
| MeSH |
Animals
Cell Line
Humans
Lactobacillus / immunology*
Lipopolysaccharide Receptors / genetics
Lipopolysaccharides / immunology*
Macrophages / cytology
Macrophages / immunology*
Macrophages / microbiology
Membrane Glycoproteins / genetics
Membrane Glycoproteins / immunology*
Membrane Glycoproteins / metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Mutant Strains
NF-kappa B / metabolism
Protoplasts / immunology
Receptors, Cell Surface / genetics
Receptors, Cell Surface / immunology*
Receptors, Cell Surface / metabolism
Spleen / cytology
Teichoic Acids / immunology*
Toll-Like Receptor 2
Toll-Like Receptors
Transfection
Tumor Necrosis Factor-alpha / immunology*
Tumor Necrosis Factor-alpha / metabolism
|
| 引用数 |
171
|
|
WOS 分野
|
INFECTIOUS DISEASES
MICROBIOLOGY
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535)
293T(RCB2202) |
