| RRC ID |
41851
|
| Author |
Akizawa Y, Nishiyama C, Hasegawa M, Maeda K, Nakahata T, Okumura K, Ra C, Ogawa H.
|
| Title |
Regulation of human FcepsilonRI beta chain gene expression by Oct-1.
|
| Journal |
Int Immunol
|
| Abstract |
The beta chain, a component of the high-affinity receptor for IgE (FcepsilonRI), plays an important role in IgE-mediated allergic reaction. The beta chain accelerates the function of FcepsilonRI by amplification of its surface expression and of signal transduction in effector cells such as mast cells and basophils. Two regulatory regions, +60/+66 and +70/+76, for the human beta chain gene that are required for the cell-type-specific transcriptional activation were identified by transient reporter assay. Electrophoretic mobility shift assay demonstrated that Oct-1 binds both the regions, among which the +70/+76 Oct-1 motif was critical for the transactivation of the beta promoter responsive to Oct-1 overexpression. Regulation of beta chain gene expression is discussed.
|
| Volume |
15(5)
|
| Pages |
549-56
|
| Published |
2003-5-1
|
| DOI |
10.1093/intimm/dxg055
|
| PMID |
12697656
|
| MeSH |
5' Flanking Region
Base Sequence
DNA-Binding Proteins / metabolism*
Electrophoretic Mobility Shift Assay
Gene Expression Regulation*
Genes, Reporter
Host Cell Factor C1
Humans
Molecular Sequence Data
Octamer Transcription Factor-1
Promoter Regions, Genetic
Receptors, IgE / biosynthesis
Receptors, IgE / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors / metabolism*
|
| IF |
3.519
|
| Times Cited |
24
|
|
WOS Category
|
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
KU812(RCB0495)
HeLa(RCB0007) |
