論文 - 詳細
| RRC ID | 41943 |
|---|---|
| 著者 | Bamba H, Ota S, Kato A, Miyatani H, Kawamoto C, Yoshida Y, Fujiwara K. |
| タイトル | Effect of rebamipide on prostaglandin receptors-mediated increase of inflammatory cytokine production by macrophages. |
| ジャーナル | Aliment Pharmacol Ther |
| Abstract |
BACKGROUND:Rebamipide (Reb) is an anti-ulcer drug, and has unique properties such as anti-inflammatory action. We previously reported that prostaglandins (PGs) dramatically increased vascular endothelial growth factor (VEGF), a known angiogenic factor and a vascular permeable factor, by activated macrophages through specific PGE receptor and peroxisome proliferator-activated receptor gamma (PPARgamma, a nuclear receptor of PG) mediated process. Effects of PGs on the production of other cytokines such as interleukin (IL)-6 and IL-8 have been controversial. AIM:To clarify the anti-inflammatory roles of Reb, we examined the effect of Reb on PGE1- and 15-deoxy-Delta12, 14-PGJ2 (a potent PPARgamma ligand, 15d-PGJ2) -induced increase of VEGF production by macrophages. Additionally, effects of these PGs on the production of IL-6 and IL-8, and modulation of these actions by Reb were studied. METHODS:Phorbol 12-myristate 13-acetate-differentiated U937 cells were used as a human macrophage model (H-Mac). VEGF, IL-6, IL-8 and cAMP were measured by EIA. RESULTS:Reb suppressed PGE1-, but not 15d-PGJ2-, induced increase of VEGF production partially through decrease of cAMP formation. Reb suppressed PGE1 -, but not 15d-PGJ2-, induced increase of IL-6 and IL-8 production. CONCLUSION:Reb suppresses membrane, but not nuclear PG receptors mediated increase of inflammatory cytokine production, which may be involved in anti-ulcer action of this drug. |
| 巻・号 | 18 Suppl 1 |
| ページ | 113-8 |
| 公開日 | 2003-7-1 |
| DOI | 10.1046/j.1365-2036.18.s1.13.x |
| PII | 1514 |
| PMID | 12925148 |
| MeSH | Alanine / analogs & derivatives* Alanine / pharmacology* Anti-Ulcer Agents / pharmacology* Cyclic AMP / metabolism Cytokines / biosynthesis* Endothelial Growth Factors / biosynthesis Humans Intercellular Signaling Peptides and Proteins / biosynthesis Interleukins / biosynthesis Lymphokines / biosynthesis Macrophages / metabolism* Quinolones / pharmacology* Receptors, Prostaglandin / drug effects Receptors, Prostaglandin / metabolism* Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| IF | 7.515 |
| 引用数 | 8 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | U-937 DE-4(RCB0425) |