論文 - 詳細
| RRC ID | 42188 |
|---|---|
| 著者 | Peng BG, Liang LJ, He Q, Huang JF, Lu MD. |
| タイトル | Expansion and activation of natural killer cells from PBMC for immunotherapy of hepatocellular carcinoma. |
| ジャーナル | World J Gastroenterol |
| Abstract |
AIM:To induce efficient expansion of natural killer (NK) cells from peripheral blood mononuclear cells (PBMCs) using a culture of anchorage-dependent Wilms tumor cell lines, and to provide a reliable supply for adoptive immunotherapy of hepatocellular carcinoma. METHODS:Culture expansion of NK cells was achieved using PBMCs cultured with Wilms tumor cells. Cytotoxicity was measured using a standard (51)Cr release assay and crystal violet staining technique. The proportions of CD3+, CD4+, CD8+, CD16+, and CD56+ cells were determined by flow cytometry. RESULTS:After PBMCs from healthy donors and hepatocellular carcinoma (HCC) were cultured with irradiated HFWT cells for 10-21 d, CD56+ CD16+ cells shared more than 50% of the cell population, and more than 80% of fresh HFWT cells were killed at an effector/target ratio of 2 over 24 h. NK-enriched lymphocyte population from HCC patients killed HCC-1 and 2 cells with sensitivities comparable to fresh TKB-17RGB cells. HCC cells proliferated 196-fold with the irradiated HFWT cells at 18 d. Stimulation by HFWT cells required intimate cell-cell interaction with PBMC. However, neither the soluble factors released from HFWT cells nor the fixed HFWT cells were effective for NK expansion. The lymphocytes expanded with IL-2 killed fresh HFWT target cells more effectively than the lymphocytes expanded with the 4-cytokine cocktail (IL-lbeta, IL-2, IL-4 and IL-6). IL-2 was the sole cytokine required for NK expansion. CONCLUSION:Wilms tumor is sensitive to human NK cells and is highly efficient for selective expansion of NK cells from PBMCs. |
| 巻・号 | 10(14) |
| ページ | 2119-23 |
| 公開日 | 2004-7-15 |
| DOI | 10.3748/wjg.v10.i14.2119 |
| PMID | 15237448 |
| PMC | PMC4572347 |
| MeSH | Animals Carcinoma, Hepatocellular / blood Carcinoma, Hepatocellular / therapy* Cell Division Cell Line, Tumor Coculture Techniques Humans Immunotherapy, Adoptive* Kidney Neoplasms / pathology* Killer Cells, Natural / cytology* Killer Cells, Natural / pathology* Liver Neoplasms / blood Liver Neoplasms / therapy* Mice Monocytes / pathology* Wilms Tumor / pathology* |
| IF | 3.665 |
| 引用数 | 11 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) HFWT(RCB0665) HMV-II(RCB0777) |