| RRC ID |
42244
|
| 著者 |
Matsumoto K, Akao Y, Yi H, Ohguchi K, Ito T, Tanaka T, Kobayashi E, Iinuma M, Nozawa Y.
|
| タイトル |
Preferential target is mitochondria in alpha-mangostin-induced apoptosis in human leukemia HL60 cells.
|
| ジャーナル |
Bioorg Med Chem
|
| Abstract |
Our previous study has shown that alpha-mangostin, a xanthone from the pericarps of mangosteen, induces caspase-3-dependent apoptosis in HL60 cells. In the current study, we investigated the mechanism of apoptosis induced by alpha-mangostin in HL60 cells. Alpha-mangostin-treated HL60 cells demonstrated caspase-9 and -3 activation but not -8, which leads us to assume that alpha-mangostin may mediate the mitochondrial pathway in the apoptosis. Parameters of mitochondrial dysfunction including swelling, loss of membrane potential (deltapsim), decrease in intracellular ATP, ROS accumulation, and cytochrome c/AIF release, were observed within 1 or 2 h after the treatment. On the other hand, alpha-mangostin-treatment did not affect expression of bcl-2 family proteins and activation of MAP kinases. These findings indicate that alpha-mangostin preferentially targets mitochondria in the early phase, resulting in indication of apoptosis in HL60 cells. Furthermore, we examined the structure-activity relationship between xanthone derivatives including alpha-mangostin and the potency of deltapsim-loss in HL60 cells. Interestingly, replacement of hydroxyl group by methoxy group remarkably decreased its potency. It was also shown that the cytotoxicity substantially correlated with deltapsim decrease. These results indicate that alpha-mangostin and its analogs would be candidates for preventive and therapeutic application for cancer treatment.
|
| 巻・号 |
12(22)
|
| ページ |
5799-806
|
| 公開日 |
2004-11-15
|
| DOI |
10.1016/j.bmc.2004.08.034
|
| PII |
S0968-0896(04)00637-6
|
| PMID |
15498656
|
| MeSH |
Apoptosis / drug effects*
Apoptosis / physiology
Cell Proliferation / drug effects
Dose-Response Relationship, Drug
Drug Delivery Systems / methods*
HL-60 Cells
Humans
Mitochondria / drug effects*
Mitochondria / physiology
Xanthones / administration & dosage*
Xanthones / chemistry
|
| IF |
3.073
|
| 引用数 |
123
|
|
WOS 分野
|
CHEMISTRY, ORGANIC
CHEMISTRY, MEDICINAL
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041) |
