| RRC ID |
42290
|
| 著者 |
Jalal Hosseinimehr S, Inanami O, Hamasu T, Takahashi M, Kashiwakura I, Asanuma T, Kuwabara M.
|
| タイトル |
Activation of c-kit by stem cell factor induces radioresistance to apoptosis through ERK-dependent expression of survivin in HL60 cells.
|
| ジャーナル |
J Radiat Res
|
| Abstract |
We investigated the effect of SCF, a c-kit ligand, on the radiosensitivity of HL60 cells. X-ray-induced apoptosis in HL60 cells was significantly lower in the presence of SCF than in the absence of SCF. This attenuation of X-ray-induced apoptosis by SCF was abolished by PD98059 (an ERK inhibitor), but not by wortmannin (a PI3-K inhibitor) or GF109203X (a PKC inhibitor). The expression of phospho-ERK1/2 (active form) and the ERK1/2-regulated expression of survivin were found to increase in cells treated with X irradiation and SCF. However, X irradiation alone induced down-regulation of the expression of phospho-ERK1/2. Our findings suggest that activation of c-kit by SCF confers radioresistance through up-regulation of ERK-dependent survivin expression in HL60 cells.
|
| 巻・号 |
45(4)
|
| ページ |
557-61
|
| 公開日 |
2004-12-1
|
| DOI |
10.1269/jrr.45.557
|
| PII |
JST.JSTAGE/jrr/45.557
|
| PMID |
15635266
|
| MeSH |
Apoptosis*
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors / pharmacology
Extracellular Signal-Regulated MAP Kinases / metabolism*
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
HL-60 Cells
Humans
Immunoblotting
Inhibitor of Apoptosis Proteins
Microtubule-Associated Proteins / biosynthesis*
Neoplasm Proteins
Proto-Oncogene Proteins c-kit / metabolism*
Radiation Tolerance
Stem Cell Factor / metabolism*
Survivin
X-Rays
|
| IF |
1.95
|
| 引用数 |
30
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041) |
