Reference - Detail
| RRC ID | 4236 |
|---|---|
| Author | Gu M, Schuske K, Watanabe S, Liu Q, Baum P, Garriga G, Jorgensen EM. |
| Title | Mu2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans. |
| Journal | J Cell Biol |
| Abstract |
Synaptic vesicles must be recycled to sustain neurotransmission, in large part via clathrin-mediated endocytosis. Clathrin is recruited to endocytic sites on the plasma membrane by the AP2 adaptor complex. The medium subunit (micro2) of AP2 binds to cargo proteins and phosphatidylinositol-4 ,5-bisphosphate on the cell surface. Here, we characterize the apm-2 gene (also called dpy-23), which encodes the only micro2 subunit in the nematode Caenorhabditis elegans. APM-2 is highly expressed in the nervous system and is localized to synapses; yet specific loss of APM-2 in neurons does not affect locomotion. In apm-2 mutants, clathrin is mislocalized at synapses, and synaptic vesicle numbers and evoked responses are reduced to 60 and 65%, respectively. Collectively, these data suggest AP2 micro2 facilitates but is not essential for synaptic vesicle recycling. |
| Volume | 183(5) |
| Pages | 881-92 |
| Published | 2008-12-1 |
| DOI | 10.1083/jcb.200806088 |
| PII | jcb.200806088 |
| PMID | 19047463 |
| PMC | PMC2592831 |
| MeSH | Adaptor Protein Complex 2 / genetics Adaptor Protein Complex 2 / metabolism* Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Clathrin / metabolism* Endocytosis* Mutation Neuromuscular Junction / metabolism* Neuromuscular Junction / ultrastructure Recombinant Fusion Proteins / metabolism Synaptic Transmission* Synaptic Vesicles / metabolism* |
| IF | 8.811 |
| Times Cited | 34 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| C.elegans | tm920 |