論文 - 詳細
| RRC ID | 42380 |
|---|---|
| 著者 | Suzuki S, Nagano T, Yamakoshi Y, Gomi K, Arai T, Fukae M, Katagiri T, Oida S. |
| タイトル | Enamel matrix derivative gel stimulates signal transduction of BMP and TGF-{beta}. |
| ジャーナル | J Dent Res |
| Abstract |
It has been shown that Emdogain Gel (Emd-Gel) containing enamel matrix proteins promotes biomineralization, such as osteogenesis and cementogenesis, during the regeneration of periodontal tissues. However, the growth factors involved in these activities of Emd-Gel remain unclear. In this study, Emd-Gel was fractionated into 22 sub-fractions by size exclusion chromatography. The osteoinductive factors, TGF-beta and BMP, were examined by a specific luciferase reporter gene assay. In the unfractionated Emd-Gel, TGF-beta-like activity was detected, while BMP activity was not. In contrast, in the fractionated Emd-Gel samples, TGF-beta-like activity was detected from fractions 8 to 13, and BMP-like activity was detected from fractions 4 to 6. Also, it was confirmed that the BMP-like activity in Emd-Gel was inhibited by authentic TGF-beta1 and TGF-beta-like activity. These results indicate that Emd-Gel contains both TGF-beta- and BMP-like growth factors that contribute to the induction of biomineralization during periodontal regeneration. |
| 巻・号 | 84(6) |
| ページ | 510-4 |
| 公開日 | 2005-6-1 |
| DOI | 10.1177/154405910508400605 |
| PII | 84/6/510 |
| PMID | 15914586 |
| MeSH | Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / antagonists & inhibitors Bone Morphogenetic Proteins / drug effects* Bone Morphogenetic Proteins / isolation & purification Cell Line Chromatography, Gel Dental Enamel Proteins / isolation & purification Dental Enamel Proteins / pharmacology* Electrophoresis, Polyacrylamide Gel Epithelial Cells Luciferases Luminescent Agents Lung / cytology Mice Mink Myoblasts Osteogenesis / drug effects Regeneration / drug effects Signal Transduction / drug effects* Transforming Growth Factor beta / drug effects* Transforming Growth Factor beta / isolation & purification Transforming Growth Factor beta / pharmacology Transforming Growth Factor beta1 |
| IF | 4.914 |
| 引用数 | 89 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |