| RRC ID |
42506
|
| 著者 |
Yamaguchi N, Argueta JG, Masuhiro Y, Kagishita M, Nonaka K, Saito T, Hanazawa S, Yamashita Y.
|
| タイトル |
Adiponectin inhibits Toll-like receptor family-induced signaling.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
Recent studies have shown that adiponectin, an adipocyte-derived cytokine, acts as a potent inhibitor of inflammatory responses. It has been also demonstrated that bacterial and viral signalings in host cells are triggered via Toll-like receptor (TLR) molecules. Therefore, in the present study, we investigated whether globular adiponectin (gAd) would be able to inhibit TLR-mediated nuclear factor-kappaB (NF-kappaB) signaling in mouse macrophages (RAW264). gAd predominantly bound to the AdipoR1 receptor and suppressed TLR-mediated NF-kappaB signaling. gAd-mediated inhibition of TLR-mediated IkappaB phosphorylation and NF-kappaB activation was eliminated by the pretreatment of cycloheximide. Also their inhibitions of gAd were blocked by preincubation of the cells with an antibody against AdipoR1, but not with an antibody against AdipoR2. Taken together, these findings indicate that adiponectin negatively regulates macrophage-like cell response to TLR ligands via an unknown endogenous product(s).
|
| 巻・号 |
579(30)
|
| ページ |
6821-6
|
| 公開日 |
2005-12-19
|
| DOI |
10.1016/j.febslet.2005.11.019
|
| PII |
S0014-5793(05)01379-7
|
| PMID |
16325814
|
| MeSH |
3T3-L1 Cells
Adipocytes / drug effects
Adiponectin / analysis
Adiponectin / genetics
Adiponectin / metabolism
Adiponectin / pharmacology
Animals
Biotinylation
Cell Line
Cycloheximide / pharmacology
Dose-Response Relationship, Drug
Genes, Reporter
Glutathione Transferase / metabolism
I-kappa B Kinase / metabolism
Luciferases / metabolism
Macrophages / drug effects
Mice
NF-kappa B / metabolism
Phosphorylation / drug effects
Protein Synthesis Inhibitors / pharmacology
Receptors, Adiponectin
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism
Recombinant Fusion Proteins / metabolism
Signal Transduction / drug effects*
Toll-Like Receptor 2 / antagonists & inhibitors*
Toll-Like Receptor 2 / metabolism
Toll-Like Receptor 4 / antagonists & inhibitors*
Toll-Like Receptor 4 / metabolism
Toll-Like Receptor 9 / antagonists & inhibitors*
Toll-Like Receptor 9 / metabolism
|
| IF |
3.057
|
| 引用数 |
182
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
