| RRC ID |
42557
|
| Author |
Tao RH, Kawate H, Wu Y, Ohnaka K, Ishizuka M, Inoue A, Hagiwara H, Takayanagi R.
|
| Title |
Testicular zinc finger protein recruits histone deacetylase 2 and suppresses the transactivation function and intranuclear foci formation of agonist-bound androgen receptor competitively with TIF2.
|
| Journal |
Mol Cell Endocrinol
|
| Abstract |
We previously reported that testicular zinc finger protein (TZF) is a corepressor for androgen receptor (AR). The present study demonstrated that a central portion (amino acids 512-663) of TZF, TZF(512-663), is responsible for both binding to AR and repressing the transactivation. TZF recruited endogenous histone deacetylase 2 (HDAC2) and formed a complex with agonist-bound AR. Imaging analyses showed that TZF and TZF(512-663) were recruited by AR and simultaneously impaired distinct AR foci formation. Quantification of the foci number using a three-dimensional imaging method revealed that the number of intranuclear AR foci was related to its transactivation activity. Moreover, increased levels of TZF dissociated a coactivator, TIF2, from the AR foci and vice versa. These results indicate that the ligand-dependent transactivation function of AR is quantitatively related to its intranuclear foci formation, and suggest that corepressors, such as TZF, act on these intranuclear events competitively with coactivators.
|
| Volume |
247(1-2)
|
| Pages |
150-65
|
| Published |
2006-3-9
|
| DOI |
10.1016/j.mce.2005.12.052
|
| PII |
S0303-7207(06)00003-7
|
| PMID |
16469430
|
| MeSH |
5-alpha-Dihydroprogesterone / pharmacology
Androgens
Animals
Cell Line
Chlorocebus aethiops
Cloning, Molecular
Histone Deacetylase 2
Histone Deacetylases / metabolism*
Humans
Male
Mice
Mutation
Nuclear Receptor Coactivator 2 / metabolism*
Protein Binding
Receptors, Androgen / metabolism*
Repressor Proteins / genetics
Repressor Proteins / metabolism*
Transcriptional Activation*
|
| IF |
3.871
|
| Times Cited |
15
|
|
WOS Category
|
ENDOCRINOLOGY & METABOLISM
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
COS-7(RCB0539) |
