| RRC ID |
42666
|
| 著者 |
Yamaguchi K, Hata K, Wada T, Moriya S, Miyagi T.
|
| タイトル |
Epidermal growth factor-induced mobilization of a ganglioside-specific sialidase (NEU3) to membrane ruffles.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Human ganglioside-specific sialidase, NEU3, localized at cell membranes is thought to regulate various biological processes at cell surfaces. We here explored functional subcellular localization of the sialidase by immunofluorescence and found accumulation at leading edges of cell membranes in the presence of serum in culture. In response to EGF, the sialidase redistributed rapidly to ruffling cell membranes of squamous carcinoma A431 cells and co-localized with Rac-1. NEU3 overexpression enhanced Rac-1 activation and cell migration as compared with controls in HeLa cells as well as in A431 cells. Consistent with co-localization with Rac-1 by immunofluorescence, NEU3 was found to co-precipitate with activated Rac bound to GST-PAK-1 fusion protein. NEU3 silencing by siRNA, in contrast, resulted in inhibition of Rac-1 activation. These results indicate that NEU3 is able to mobilize to membrane ruffles in response to growth stimuli and activate the Rac-1 signaling by co-localization with Rac-1, leading to increased cell motility.
|
| 巻・号 |
346(2)
|
| ページ |
484-90
|
| 公開日 |
2006-7-28
|
| DOI |
10.1016/j.bbrc.2006.05.136
|
| PII |
S0006-291X(06)01196-X
|
| PMID |
16765317
|
| MeSH |
Animals
Cell Line
Cell Membrane Structures / metabolism*
Cell Movement
Chlorocebus aethiops
Enzyme Activation
Epidermal Growth Factor / physiology*
Humans
Mice
Neuraminidase / biosynthesis
Neuraminidase / genetics
Neuraminidase / metabolism*
Protein Binding
Protein Serine-Threonine Kinases / metabolism
Protein Transport
p21-Activated Kinases
rac1 GTP-Binding Protein / metabolism
|
| IF |
2.985
|
| 引用数 |
39
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-1(RCB0143) |
