| RRC ID |
42697
|
| 著者 |
Huitema LF, van Weeren PR, Barneveld A, van de Lest CH, Helms JB, Vaandrager AB.
|
| タイトル |
Iron ions derived from the nitric oxide donor sodium nitroprusside inhibit mineralization.
|
| ジャーナル |
Eur J Pharmacol
|
| Abstract |
Sodium nitroprusside (SNP) is a nitric oxide (NO) donor drug, which is therapeutically used as a vasodilating drug in heart transplantations. In our previous study it was found that SNP at a concentration of 100 microM inhibited mineralization in a cell culture system, indicating that the beneficial effects of this drug may also include inhibition of vascular calcification. The aim of this study was to investigate which bioactive compounds generated from SNP inhibit mineralization. ATDC5 cells were grown for 14 days and mineralization was induced by addition of 5 mM phosphate for 24 h. Mineralization was determined by staining precipitated calcium with an alizarin red stain. It was found that the NO donors S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine were not able to inhibit mineralization and NO scavengers could not antagonize the inhibiting effect of SNP on mineralization. The iron chelator deferoxamine (200 microM) antagonized the inhibiting effect on mineralization mediated by SNP and ammonium iron sulfate inhibited mineralization in a dose-dependent manner (10-100 microM). Furthermore, iron ions (30 microM) were detected to be released from SNP in the cell culture. These data show that the iron moiety of sodium nitroprusside, rather than nitric oxide inhibits mineralization.
|
| 巻・号 |
542(1-3)
|
| ページ |
48-53
|
| 公開日 |
2006-8-7
|
| DOI |
10.1016/j.ejphar.2006.06.006
|
| PII |
S0014-2999(06)00621-2
|
| PMID |
16828741
|
| MeSH |
Animals
Bepridil / pharmacology
Calcium / analysis
Calcium / metabolism
Catalase / pharmacology
Cell Line
Cyclic N-Oxides / pharmacology
Dose-Response Relationship, Drug
Ferric Compounds / pharmacology
Ferricyanides / pharmacology
Ferrous Compounds / pharmacology
Free Radical Scavengers / pharmacology
Imidazoles / pharmacology
Iron / chemistry
Iron / pharmacology*
Mannitol / pharmacology
Minerals / metabolism*
Molecular Structure
Nitric Oxide / antagonists & inhibitors
Nitric Oxide Donors / chemistry
Nitric Oxide Donors / pharmacology*
Nitroprusside / chemistry
Nitroprusside / pharmacology*
Oxidation-Reduction
Penicillamine / analogs & derivatives
Penicillamine / pharmacology
Quaternary Ammonium Compounds / pharmacology
Reactive Oxygen Species / antagonists & inhibitors
S-Nitrosoglutathione / pharmacology
Superoxide Dismutase / pharmacology
|
| IF |
3.263
|
| 引用数 |
8
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
