| RRC ID |
42953
|
| 著者 |
Sawada N, Taketani Y, Amizuka N, Ichikawa M, Ogawa C, Nomoto K, Nashiki K, Sato T, Arai H, Isshiki M, Segawa H, Yamamoto H, Miyamoto K, Takeda E.
|
| タイトル |
Caveolin-1 in extracellular matrix vesicles secreted from osteoblasts.
|
| ジャーナル |
Bone
|
| Abstract |
Caveolin-1 is an essential and signature protein of caveolae, which are small invaginations of the plasma membrane enriched in cholesterol and sphingolipids. Although high levels of expression of caveolin-1 have been demonstrated in osteoblasts as well as endothelial cells, fibroblasts, and muscular cells, the role of caveolin-1 in osteoblasts has not been clarified. Here, we show that caveolin-1 is secreted from osteoblasts in the form of matrix vesicles; extracellular vesicles released from the plasma membrane of osteoblasts. In this study, caveolae and matrix vesicles were similarly enriched in cholesterol and sphingomyelin in fractions isolated from mineralizing MC3T3-E1 cells. Interestingly, in the MC3T3-E1 cells caveolin-1 was enriched in the matrix vesicle fraction as well as the caveolar membrane fraction, and the amount of caveolin-1 in the matrix vesicle fraction increased as differentiation progressed. Localization of caveolin-1 in matrix vesicles was also confirmed in murine tibia. Furthermore, overexpression of caveolin-1 enhanced matrix calcification in MC3T3-E1 cells, whereas knockdown of caveolin-1 diminished it. These results suggest that secreted caveolin-1 as a component of matrix vesicles may play an important role in osteoblast calcification.
|
| 巻・号 |
41(1)
|
| ページ |
52-8
|
| 公開日 |
2007-7-1
|
| DOI |
10.1016/j.bone.2007.02.030
|
| PII |
S8756-3282(07)00101-9
|
| PMID |
17448744
|
| MeSH |
3T3 Cells
Alkaline Phosphatase / metabolism
Animals
Base Sequence
Calcification, Physiologic
Caveolin 1 / antagonists & inhibitors
Caveolin 1 / genetics
Caveolin 1 / metabolism*
Cell Differentiation
Cholesterol / metabolism
DNA Primers / genetics
Extracellular Matrix / metabolism
Gene Expression
Mice
Microscopy, Immunoelectron
Osteoblasts / metabolism*
Osteoblasts / ultrastructure
Phosphates / metabolism
RNA Interference
Secretory Vesicles / metabolism
Sphingomyelins / metabolism
Tibia / metabolism
Tibia / ultrastructure
|
| IF |
4.147
|
| 引用数 |
20
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
