| RRC ID |
42989
|
| Author |
Zhao S, Zhang J, Zhang X, Dong X, Sun X.
|
| Title |
Arsenic trioxide induces different gene expression profiles of genes related to growth and apoptosis in glioma cells dependent on the p53 status.
|
| Journal |
Mol Biol Rep
|
| Abstract |
We have previously reported that As(2)O(3) affected cell cycle progression and cyclins D1 and B1 expression in two glioma cell lines differing in p53 status (U87MG-wt; T98G-mutated). In the present study, we further demonstrated that As(2)O(3) affected proliferation, viability and apoptosis of the two cell lines in a dose- and time-dependent manner, and T98G cells were more sensitive than U87MG cells to As(2)O(3) -induced apoptosis and inhibition of proliferation and viability. We further investigated the expression profiles of genes related with apoptosis and cell cycle in the two cell lines with a human cDNA-microarray (SuperArray) spotted with 267 genes of apoptosis and cell cycle. Thirty five genes were upregulated and 15 genes downregulated at least 2-fold by As(2)O(3) in U87-MG cells; whereas, 38 genes were upregulated and 21 genes downregulated at least 2-fold in T98G cells by As(2)O(3). After As(2)O(3) treatment, p53 expression was upregulated 56.5-fold in T98G cells, but only 6.0-fold in U87MG cells. The results indicate that As(2)O(3) suppresses the growth of U87MG cells mainly by regulating expression of genes of cell cycle arrest, stress and toxicity; whereas As(2)O(3) affects T98G cells mainly by regulating expression of genes belonging to Bcl-2, tumor necrotic factor receptor and ligand families. The data may be helpful for optimizing As(2)O(3) as an anti-cancer drug in the treatment of gliomas.
|
| Volume |
35(3)
|
| Pages |
421-9
|
| Published |
2008-9-1
|
| DOI |
10.1007/s11033-007-9102-6
|
| PMID |
17530438
|
| MeSH |
Apoptosis / drug effects*
Arsenic Trioxide
Arsenicals / pharmacology*
Cell Line, Tumor
Gene Expression Profiling
Gene Expression Regulation, Neoplastic / drug effects*
Glioma / genetics*
Glioma / pathology*
Humans
Oxides / pharmacology*
RNA, Messenger / genetics
Tumor Suppressor Protein p53 / genetics*
|
| IF |
1.402
|
| Times Cited |
33
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
U-87 MG(RCB0419)
T98G(RCB1954) |
