RRC ID |
43015
|
著者 |
Aoki T, Hagiwara H, Matsuzaki T, Suzuki T, Takata K.
|
タイトル |
Internalization of caveolae and their relationship with endosomes in cultured human and mouse endothelial cells.
|
ジャーナル |
Anat Sci Int
|
Abstract |
Treatment of cells with pervanadate or vanadate induces the phosphorylation of caveolin-1 and its internalization from the cell surface, but the intracellular fate of caveolae has not been fully elucidated. In the present study, we examined the fate of endocytosed caveolae in human umbilical vein endothelial cells and mouse endothelial KOP2.16 cells. The localization of internalized caveolae and their relationship with the endosomes were examined by immunofluorescence microscopy as well as by immunoprecipitation and chasing of biotinylated transferrin. In untreated cells, caveolin-1 was mostly confined to the cell surface. When cells were treated with either pervanadate for 30 min or vanadate for 3 h, many caveolin-1-labeled vesicles were formed inside the cells, some of which were colocalized with Rab5 or Rab4. The internalized caveolin-1 was colocalized with the endocytosed transferrin in the Rab5-, Rab4- or early endosome antigen-1-labeled compartment where caveolin-1 was phosphorylated. It then moved to the Rabl 1-associated compartment. Immunogold electron microscopy revealed that internalized caveolin-1 colocalized with Rab5 or Rab4 in vesicles larger than caveolae. These results suggest that the internalized caveolae interact with early endosomes.
|
巻・号 |
82(2)
|
ページ |
82-97
|
公開日 |
2007-6-1
|
DOI |
10.1111/j.1447-073X.2006.00160.x
|
PMID |
17585564
|
MeSH |
Animals
Biological Transport / physiology
Blotting, Western
Caveolin 1 / metabolism*
Cells, Cultured
Endosomes / metabolism*
Endosomes / ultrastructure
Endothelial Cells / cytology*
Humans
Immunohistochemistry
Immunoprecipitation
Mice
Microscopy, Electron
Microscopy, Fluorescence
Phosphorylation
Umbilical Veins / cytology
Vanadates
rab4 GTP-Binding Proteins / metabolism
rab5 GTP-Binding Proteins / metabolism
|
IF |
1.512
|
引用数 |
9
|
WOS 分野
|
ANATOMY & MORPHOLOGY
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
SR-3Y1(RCB0353) |