論文 - 詳細
RRC ID | 4306 |
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著者 | Tan FJ, Husain M, Manlandro CM, Koppenol M, Fire AZ, Hill RB. |
タイトル | CED-9 and mitochondrial homeostasis in C. elegans muscle. |
ジャーナル | J Cell Sci |
Abstract |
Mitochondrial homeostasis reflects a dynamic balance between membrane fission and fusion events thought essential for mitochondrial function. We report here that altered expression of the C. elegans BCL2 homolog CED-9 affects both mitochondrial fission and fusion. Although striated muscle cells lacking CED-9 have no alteration in mitochondrial size or ultrastructure, these cells appear more sensitive to mitochondrial fragmentation. By contrast, increased CED-9 expression in these cells produces highly interconnected mitochondria. This mitochondrial phenotype is partially suppressed by increased expression of the dynamin-related GTPase DRP-1, with suppression dependent on the BH3 binding pocket of CED-9. This suppression suggests that CED-9 directly regulates DRP-1, a model supported by our finding that CED-9 activates the GTPase activity of human DRP1. Thus, CED-9 is capable of regulating the mitochondrial fission-fusion cycle but is not essential for either fission or fusion. |
巻・号 | 121(Pt 20) |
ページ | 3373-82 |
公開日 | 2008-10-15 |
DOI | 10.1242/jcs.032904 |
PII | jcs.032904 |
PMID | 18827010 |
PMC | PMC2785848 |
MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Dynamins GTP Phosphohydrolases / genetics GTP Phosphohydrolases / metabolism Homeostasis / physiology* Humans Membrane Fusion / physiology* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Mitochondria / genetics Mitochondria / metabolism* Mitochondrial Membranes / metabolism* Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Models, Biological Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / metabolism* |
IF | 4.573 |
引用数 | 27 |
WOS 分野 | CELL BIOLOGY |
リソース情報 | |
線虫 | tm1133 |