論文 - 詳細
RRC ID | 43140 |
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著者 | Yanagisawa S, Zhu Z, Kobayashi I, Uchino K, Tamada Y, Tamura T, Asakura T. |
タイトル | Improving cell-adhesive properties of recombinant Bombyx mori silk by incorporation of collagen or fibronectin derived peptides produced by transgenic silkworms. |
ジャーナル | Biomacromolecules |
Abstract |
Silk of Bombyx mori can be used as various biomaterials. Especially, it is useful as a protein for coating the surface of cell culture plates since the silk possesses a biocompatibility to the cultured cells. However, the cell-adhesive ability is weaker than collagen or fibronectin, which are used for coating the plate more frequently (Yao et al. J. Biochem., 2004, 136, 643-649). To increase the biocompatibility of the silk, we constructed transgenic silkworms, inserting the modified fibroin light-chain genes for making recombinant silks that possessed partial collagen or fibronectin sequences, that is, [GERGDLGPQGIAGQRGVV(GER)3GAS]8GPPGPCCGGG or [TGRGDSPAS]8, respectively. Films were made from the recombinant silks, and the cell-adhesive activity for cultured mammalian cells was observed. The results showed that the two types of recombinant silk films possessed a much higher cell-adhesive activity as compared to the original unmodified silk. Especially, the recombinant silk with the sequence [TGRGDSPAS]8, produced by a transgenic Nd-sD mutant, gave a 6 times higher activity than the original unmodified silk. |
巻・号 | 8(11) |
ページ | 3487-92 |
公開日 | 2007-11-1 |
DOI | 10.1021/bm700646f |
PMID | 17939714 |
MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Bombyx / chemistry Bombyx / genetics Bombyx / metabolism* Cell Adhesion / drug effects Cell Line Collagen / chemistry Collagen / genetics Collagen / metabolism* Collagen / pharmacology* Escherichia coli / genetics Escherichia coli / metabolism Fibronectins / chemistry Fibronectins / genetics Fibronectins / metabolism* Fibronectins / pharmacology* Mice Molecular Sequence Data Mutation / genetics Oligopeptides / genetics Oligopeptides / metabolism Oligopeptides / pharmacology Peptide Fragments / chemistry Peptide Fragments / genetics Peptide Fragments / metabolism* Peptide Fragments / pharmacology Recombinant Proteins / genetics Recombinant Proteins / metabolism Silk / genetics Silk / metabolism* |
IF | 6.092 |
引用数 | 76 |
WOS 分野 | POLYMER SCIENCE CHEMISTRY, ORGANIC BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | BALB/3T3 clone A31(RCB0005) |