RRC ID |
43411
|
著者 |
Suzuki T, Ishigami Y, Okada N, Kaneko A, Fukutomi R, Isemura M.
|
タイトル |
Differentiation-associated alteration in gene expression of importins and exportins in human leukemia HL-60 cells.
|
ジャーナル |
Biomed Res
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Abstract |
Employing the DNA microarray technique, we previously reported the alteration in gene expression of nucleocytoplasmic transport factors, importins and exportins, induced by 1,25-dihydroxyvitamin D3 (DVD) in human leukemia HL-60 cells. Here, we used the quantitative reverse transcription-polymerase chain reaction method to confirm such previous findings, and compared them with those from the cells treated with all-trans-retinoic acid (ATRA). The results indicated that the gene expression of the transport factors examined was mostly down-regulated following differentiation induced by DVD and ATRA, but importin alpha5 gene expression was up-regulated in either case. The differences were found in the gene expression of importin alpha3 and exportin 6 between the cells after treatments with DVD and ATRA. These variations may be related to the difference between HL-60 cell lineages differentiating into monocytes/macrophages and granulocytes. The present findings provide further evidence to support the important roles of importins and exportins in cell differentiation.
|
巻・号 |
29(3)
|
ページ |
141-5
|
公開日 |
2008-6-1
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DOI |
10.2220/biomedres.29.141
|
PII |
JST.JSTAGE/biomedres/29.141
|
PMID |
18614847
|
MeSH |
Base Sequence
Calcitriol / pharmacology
DNA Primers
Gene Expression*
HL-60 Cells
Humans
Karyopherins / metabolism*
Leukemia, Promyelocytic, Acute / genetics
Leukemia, Promyelocytic, Acute / metabolism*
Leukemia, Promyelocytic, Acute / pathology
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Tretinoin / pharmacology
|
IF |
1.429
|
引用数 |
19
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
リソース情報 |
ヒト・動物細胞 |
|