論文 - 詳細
RRC ID | 43617 |
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著者 | Tamagawa K, Horiuchi T, Wada T, Bannai K, Ando T. |
タイトル | Polysaccharide-K (PSK) may suppress surgical stress-induced metastasis in rat colon cancer. |
ジャーナル | Langenbecks Arch Surg |
Abstract |
PURPOSE:We previously demonstrated that hepatic ischemia and reperfusion (I/R) injury increased liver metastasis and cancer growth of RCN-H4 cells. Using a rat model of hepatic I/R-induced liver metastasis, we investigated the metastasis-suppressing effect of polysaccharide-K (PSK), a biological response modifier composed of protein-bound polysaccharide. METHODS:Fischer rats underwent 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. PSK was administered orally before I/R, after I/R, or before and after I/R. The weights of metastatic lesions of the liver or the numbers of liver metastatic nodules were determined on day 21. The effect of PSK on angiogenesis was studied by a rat cornea model using RCN-H4 cells or a vascular endothelial growth factor (VEGF)-containing pellet and an in vitro VEGF-induced endothelial cell migration assay. RESULTS:PSK administration significantly (p < 0.05) suppressed the I/R-induced increase in hepatic metastasis of RCN-H4 cells. The suppression of I/R-promoted metastasis was observed irrespective of the timing of administration. Furthermore, PSK significantly suppressed angiogenesis induced by RCN-H4 cells (p < 0.05) and the VEGF pellet (p < 0.01). PSK significantly suppressed the VEGF-induced migration of vascular endothelial cells (p < 0.05). CONCLUSION:PSK may suppress metastasis induced by hepatic I/R. The suppression of angiogenesis by PSK may be one of the mechanisms of the inhibition of hepatic metastasis. |
巻・号 | 397(3) |
ページ | 475-80 |
公開日 | 2012-3-1 |
DOI | 10.1007/s00423-011-0896-2 |
PMID | 22207390 |
MeSH | Animals Cell Line, Tumor Colonic Neoplasms / pathology* Disease Models, Animal Immunologic Factors / therapeutic use* Liver Neoplasms / physiopathology Liver Neoplasms / secondary Male Neovascularization, Physiologic / drug effects* Proteoglycans / therapeutic use* Rats Rats, Inbred F344 Reperfusion Injury / physiopathology Stress, Physiological / drug effects* Stress, Physiological / physiology Vascular Endothelial Growth Factor A / metabolism |
IF | 2.184 |
引用数 | 5 |
WOS 分野 | SURGERY |
リソース情報 | |
ヒト・動物細胞 | RCN-H-4(RCB0512) |