| RRC ID |
43754
|
| 著者 |
Li Y, Mitsuhashi S, Ikejo M, Miura N, Kawamura T, Hamakubo T, Ubukata M.
|
| タイトル |
Relationship between ATM and ribosomal protein S6 revealed by the chemical inhibition of Ser/Thr protein phosphatase type 1.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
The optimal cellular responses to DNA damage are modulated by kinase and phosphatase. The ataxia telangiectasia mutated (ATM) is a Ser/Thr kinase which is the core of the DNA damage signaling apparatus. The Ser/Thr protein phosphatase type 1 (PP1) inhibitor, tautomycetin (TC) and an antibody to the phospho-(S/T)Q sites of the ATM substrate were used to identify the common substrates for PP1 and ATM in regulating the pathway for DNA damage response. Ribosomal protein S6 (RPS6) was first identified as a substrate for PP1 and ATM. The phosphorylation at Ser247 of RPS6 was then significantly decreased by PP1-mediated dephosphorylation immediately after UV irradiation. These results suggest that PP1 specifically dephosphorylated RPS6 at phospho-Ser247 in vivo. In response to DNA damage, ATM activity was finally required for the phosphorylation of RPS6 at Ser247. We propose from these results a novel mechanism for modulating the RPS6 function by PP1 and ATM which regulates cell growth and survival in response to DNA-damage stimuli.
|
| 巻・号 |
76(3)
|
| ページ |
486-94
|
| 公開日 |
2012-1-1
|
| DOI |
10.1271/bbb.110774
|
| PII |
JST.JSTAGE/bbb/110774
|
| PMID |
22451389
|
| MeSH |
Amino Acid Motifs
Antibody Specificity
Ataxia Telangiectasia Mutated Proteins
Cell Cycle Proteins / metabolism*
DNA Damage
DNA-Binding Proteins / metabolism*
Enzyme Inhibitors / pharmacology*
Furans / pharmacology
HEK293 Cells
HeLa Cells
Humans
Lipids / pharmacology
Models, Molecular
Phosphorylation / drug effects
Phosphorylation / radiation effects
Protein Conformation
Protein Phosphatase 1 / antagonists & inhibitors*
Protein Phosphatase 1 / chemistry
Protein Phosphatase 1 / immunology
Protein Phosphatase 1 / metabolism*
Protein Serine-Threonine Kinases / metabolism*
Ribosomal Protein S6 / metabolism*
Signal Transduction / drug effects
Signal Transduction / radiation effects
Tumor Suppressor Proteins / metabolism*
Ultraviolet Rays
|
| IF |
1.516
|
| 引用数 |
9
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
293T(RCB2202) |
